Single cell multi-omics sequencing platform to understand the building blocks of life.

单细胞多组学测序平台可了解生命的组成部分。

• 批准号: BB/W019493/1
• 负责人: Sonja Vernes
• 金额: $ 36.71万
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW019493%2F1
• 关键词: Single; cell; multi; omics; sequencing

It is necessary to clearly resolve and describe living systems at a cellular resolution, in order to understand the rules of life, to understand health and increase healthy lifespans, or to improve sustainability in agriculture. This presents a major challenge as organisms are often composed of huge numbers of cells with very different properties that have complex interactions with each other, and until recently there was no technology that would allow such resolution. This single cell multi-omics phenotyping platform allows cells from any tissue or organism to be explored at a single cell level. The platform makes it possible to take a large collection of cells (e.g. a tissue sample, biopsies, cells treated with specific compounds) and give each cell a unique barcode label before determining its properties. Each cell can then be explored for a range of molecular properties such as determining the sequence or structure of the genome, the genes that are expressed in the cell, and the proteins expressed in the cell or at the surface of the cell.The proposed platform will allow researchers to explore the intricate cellular architecture that underlies how traits are determined in complex organisms, and shed light on how they evolved. The range of research facilitated by this platform addresses questions including: how communication behaviour is encoded in the brain; how the body controls motor functions and what goes wrong in motor neuron disease; how genetic mechanisms influence development, fertility and parental provisioning; how organisms adapt to extreme conditions over evolution; neural control of nest building in birds; the role of genome structure in directing the forms tissues or animal bodies take; cancer progression and the effect of cancer drugs on the body; how the immune system functions in health and disease; normal and abnormal neurodevelopment; improving crop production; and developing new computational methods for data analyses. This cutting-edge technology platform would therefore facilitate a massive step forward in a diverse range of key research areas, that will impact crucial research fields across evolution, development, physiology, behaviour, medicine, agriculture and environmental sustainability.

有必要以细胞分辨率清晰地解析和描述生命系统，以了解生命的规则，了解健康并增加健康寿命，或提高农业的可持续性。这提出了一个重大挑战，因为生物体通常由大量具有非常不同特性的细胞组成，这些细胞彼此之间具有复杂的相互作用，直到最近才有技术可以实现这种解决方案。这种单细胞多组学表型分析平台允许在单细胞水平上探索来自任何组织或生物体的细胞。该平台可以采集大量细胞（例如组织样本、活检、用特定化合物处理的细胞），并在确定其特性之前为每个细胞提供独特的条形码标签。每个细胞都可以探索一系列的分子特性，例如确定基因组的序列或结构，细胞中表达的基因，以及细胞中或细胞表面表达的蛋白质。拟议的平台将允许研究人员探索复杂的细胞结构，这些结构是复杂生物体中性状如何确定的基础，并阐明它们是如何进化的。该平台促进的研究范围涉及的问题包括：沟通行为如何在大脑中编码;身体如何控制运动功能以及运动神经元疾病中出现了什么问题;遗传机制如何影响发育，生育力和父母供应;生物体如何适应进化过程中的极端条件;鸟类筑巢的神经控制;基因组结构在指导组织或动物身体形成中的作用;癌症进展和抗癌药物对身体的影响;免疫系统在健康和疾病中的作用;正常和异常的神经发育;提高作物产量;以及开发新的数据分析计算方法。因此，这一尖端技术平台将促进在各种关键研究领域向前迈出一大步，这将影响进化、发展、生理学、行为、医学、农业和环境可持续性等关键研究领域。