IRON-APOFERRITIN--EFFECT & STRUCTURE OF SUBUNIT DIMERS
IRON-APOFERRITIN--EFFECT & STRUCTURE OF SUBUNIT DIMERS
批准号:
3286062
负责人:
ELIZABETH C THEIL
金额:
$9.16万
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-08-01 至 1989-07-31
中文摘要
铁蛋白是一种金属蛋白,它以生物可利用的形式储存铁,
英文摘要
Ferritin, a metalloprotein which stores iron in a bioavailable form,
overcomes the low solubility (10-18M) of iron in living systems. Small
amounts of ferritin occur in all cells of higher organisms, providing iron
for proteins of DNA synthesis, electron transport, and oxygen activation
and transport, while large amounts occur in specialized cells of iron
storage, e.g. liver, spleen, and red cells of embryo. Pathological
conditions occur when iron stores are low or are overloaded.
Superimposed on a highly conserved sequence are cell-specific features of
ferritin sequence and structure which may relate to function and/or
regulation. Ferritin structure could be further modified by cytoplasmic
components leading to functional changes. We have recently identified such
a change: ferritin subunit dimer crosslinks, a posttranslational
modification of ferritin, appear to regulate the iron content of ferritin
in vivo. The observation is the first identified, natural structural
modification related to the iron storage function of ferritin, to our
knowledge. Crosslinking with F2DNB replicates the effect. The structural
requirements for crosslinks (which are not -S-S) will be examined in terms
of the identity of the linked amino acids, the sequence and subunit
restrictions (by sequence analysis and comparison to known subunit
sequences), the sequences recognized by monoclonal antibodies sensitive to
the presence of crosslinks, the biosynthetic pathway of crosslink formation
(H-3-leucine pulse labeling) and cytoplasmic components which may influence
crosslinks (transpeptidases? ascorbate?). Cloned ferritin cDNA from lamb
spleen or other sources will be prepared and used to gain information about
the sequence and regulation of ferritin mRNA related to crosslinks. The
functional effect of crosslinks will be measured as the effect of
crosslink-sensitive monoclonal antibodies on iron uptake and release in
vitro and as the effect of crosslinks on Fe-protein interactions,
phosphate-Fe-protein interactions, and iron core structure, using X-ray
absorption spectroscopy (EXAFS and XANES). The results will be important
in understanding the molecular basis for pathologically altered iron
storage, e.g. iron deficiency anemia, hemochromatosis, thalassemia, and
cirrhosis, as well as understanding the relationship among cell-specific
features of protein structure, cytoplasmic modifying agents, and regulation
of function.
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会议论文
Workshop on BioIron in Thalassemia, Sickle Cell Disease and Hemochromatosis
-
批准号:6702966
-
项目类别:
-
资助金额:$2.55万
-
财政年份:2004
-
负责人:ELIZABETH C THEIL
-
依托单位:
Workshop on Biolron in Thalassemia & Sickle Cell Disease
-
批准号:6555013
-
项目类别:
-
资助金额:$1.45万
-
财政年份:2002
-
负责人:ELIZABETH C THEIL
-
依托单位:
IRON & OXYGEN BIOLOGY IN SCD, HH & BETA-THAL MICE
-
批准号:6381947
-
项目类别:
-
资助金额:$19.34万
-
财政年份:2000
-
负责人:ELIZABETH C THEIL
-
依托单位:
IRON & OXYGEN BIOLOGY IN SCH, HH & BETA-THAL MICE
-
批准号:6258562
-
项目类别:
-
资助金额:$19.34万
-
财政年份:2000
-
负责人:ELIZABETH C THEIL
-
依托单位:
IRON & OXYGEN BIOLOGY IN SCD, HH & BETA-THAL MICE
-
批准号:6517855
-
项目类别:
-
资助金额:$19.34万
-
财政年份:2000
-
负责人:ELIZABETH C THEIL
-
依托单位:
3 DIMENSIONAL STRUCTURE OF IRE IN FERRITIN MRNA, STUDIED BY NMR SPECTROSCOPY
-
批准号:6309021
-
项目类别:
-
资助金额:$2.74万
-
财政年份:2000
-
负责人:ELIZABETH C THEIL
-
依托单位:
Ferritin and Iron Nutrition in Health and Disease
-
批准号:6436336
-
项目类别:
-
资助金额:$34.77万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
FERRITIN AND IRON DEFICIENCY--A NEW LOOK AT THE PROBLEM
-
批准号:2901238
-
项目类别:
-
资助金额:$13.16万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
FERRITIN AND IRON DEFICIENCY--A NEW LOOK AT THE PROBLEM
-
批准号:2797653
-
项目类别:
-
资助金额:$13.78万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
FERRITIN AND IRON DEFICIENCY--A NEW LOOK AT THE PROBLEM
-
批准号:6184363
-
项目类别:
-
资助金额:$13.15万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
Ferritin and Iron Nutrition in Health and Disease
-
批准号:6686411
-
项目类别:
-
资助金额:$36.03万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
3 DIMENSIONAL STRUCTURE OF IRE IN FERRITIN MRNA, STUDIED BY NMR SPECTROSCOPY
-
批准号:6281479
-
项目类别:
-
资助金额:$5.83万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
Ferritin and Iron Nutrition in Health and Disease
-
批准号:6821367
-
项目类别:
-
资助金额:$37.01万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
Ferritin and Iron Nutrition in Health and Disease
-
批准号:6621749
-
项目类别:
-
资助金额:$34.2万
-
财政年份:1998
-
负责人:ELIZABETH C THEIL
-
依托单位:
3 DIMENSIONAL STRUCT OF IRE IN FERRITIN MRNA, STUDIED BY NMR SPECTROSCOPY
-
批准号:6251979
-
项目类别:
-
资助金额:$1.96万
-
财政年份:1997
-
负责人:ELIZABETH C THEIL
-
依托单位:
INTERNATIONAL CONFERENCE ON BIOLRON
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批准号:2150353
-
项目类别:
-
资助金额:$1.5万
-
财政年份:1995
-
负责人:ELIZABETH C THEIL
-
依托单位:
METALS IN BIOLOGY GORDON CONFERENCE
-
批准号:2190539
-
项目类别:
-
资助金额:$0.6万
-
财政年份:1995
-
负责人:ELIZABETH C THEIL
-
依托单位:
IRON-APOFERRITIN--EFFECT & STRUCTURE OF SUBUNIT DIMERS
-
批准号:3286059
-
项目类别:
-
资助金额:$9.65万
-
财政年份:1985
-
负责人:ELIZABETH C THEIL
-
依托单位:
IRON-APOFERRITIN--EFFECT & STRUCTURE OF SUBUNIT DIMERS
-
批准号:3286064
-
项目类别:
-
资助金额:$11.04万
-
财政年份:1985
-
负责人:ELIZABETH C THEIL
-
依托单位:
IRON-APOFERRITIN--EFFECT & STRUCTURE OF SUBUNIT DIMERS
-
批准号:3286063
-
项目类别:
-
资助金额:$9.69万
-
财政年份:1985
-
负责人:ELIZABETH C THEIL
-
依托单位:
海外基金