FERRITIN AND IRON DEFICIENCY--A NEW LOOK AT THE PROBLEM

铁蛋白和缺铁——对问题的新看法

• 批准号: 6184363
• 负责人: ELIZABETH C THEIL
• 金额: $ 13.15万
• 依托单位: CHILDREN'S HOSPITAL & RES CTR AT OAKLAND
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-18 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6184363
• 关键词: ascorbate; clinical research; dietary iron; ferritin; food; food processing /preparation; gastrointestinal nutrient absorption; human subject; inositol phosphates; microcytic /hypochromic anemia; nutrient bioavailability; nutrient intake activity; nutrition related tag; radiotracer; soybeans

A sustainable solution to dietary iron deficiency can be achieved in part by increasing bioavailable iron in seeds used for food such as soybeans. Dietary iron deficiency and anemia afflict 1.5 billion people world-wide, approximately 6.6 percent of women of reproductive age and approximately 11 percent of children and adolescents in the U.S. are affected. Since ferritin is used as a natural source of iron in the early development of humans, other animals, and plants, the availability of iron in soybean seeds that are rich in ferritin was tested in iron deficient rats. Soybeans were selected for study because of the naturally high iron levels, a variety of readily accessible cultivars, the large consumption world-wide including, recently, in the United States. Measuring bioavailability of ferritin iron has recently shown that horse spleen ferritin and soybean meal ferritin from iron-rich soybean cultivars can cure nutritional iron deficiency at amounts of iron equivalent to ferrous sulfate. Diets were equivalent for carbohydrate, protein, and fat. Recovery from anemia was measured by hematocrit, hemoglobin concentration, and tissue (spleen, liver, brain) iron concentrations. The results contrast with those obtained previously with labelled Fe, in part because of new knowledge which shows (a) a slow equilibration of the isotopically labeled iron (1-2 years); (b) coexistence of ferritin with in vivo in pools with distinctive labeling; (c) that extrinsically added iron labels only approximately 0.1 percent of the ferritin iron; and (d) that protocols for intrinsically labelled iron likely produced a stress ferritin which can have slow iron turnover. Preliminary data show that soybean seed ferritin concentrations are cultivar specific, that nodule iron is recycled to provide approximately 41 percent seed iron, accounting at least in part for the high concentration of iron in legume seeds, and that much of the iron in soybean seeds is in ferritin. In order to further understand the relationship between seed ferritin and bioavailable iron, experiments in iron-deficient and iron-sufficient humans are proposed to analyze: (1) The utilization of soybean ferritin iron from soy flour or tofu or broth; the effect of ascorbate and phytate on absorption will be determined as well to evaluate the influence of inhibitors and enhancers. (2) The effect of soybean processing on retention of soybean ferritin. If time permits, the ferritin promotor and seed ferritin will be analyzed in crosses of cultivars with high and low amounts of ferritin and soluble iron to allow future development of soybeans with enhanced amounts of iron that can contribute to a sustainable solution to iron deficiency in humans.

可以通过以下方式实现膳食缺铁的可持续解决方案： 部分是通过增加用于食品的种子中的生物可利用铁，例如 大豆。 膳食缺铁和贫血困扰着 15 亿人 在世界范围内，大约 6.6% 的育龄妇女 美国大约 11% 的儿童和青少年 做作的。 由于铁蛋白被用作铁的天然来源 人类、其他动物和植物的早期发育，可用性 测试了富含铁蛋白的大豆种子中的铁含量 缺乏的老鼠。 选择大豆进行研究是因为 天然高铁含量，各种容易获得的品种， 全球范围内的大量消费，包括最近在美国 国家。 最近测量铁蛋白的生物利用度表明 那马脾铁蛋白和豆粕铁蛋白富含铁 大豆品种可以治疗营养性铁缺乏症 铁相当于硫酸亚铁。 饮食相当于 碳水化合物、蛋白质和脂肪。 贫血的恢复情况通过以下方法测量 血细胞比容、血红蛋白浓度和组织（脾、肝、脑） 铁浓度。 结果与获得的结果形成对比 以前用标记的铁，部分原因是新知识 显示 (a) 同位素标记的铁 (1-2 年）; (b) 铁蛋白与体内共存于池中 独特的标签； (c) 仅外在添加铁标签 大约 0.1% 的铁蛋白； (d) 该议定书 因为内在标记的铁可能产生应激铁蛋白 这可能导致铁周转缓慢。 初步数据显示，大豆 种子铁蛋白浓度因品种而异，球铁是 回收后可提供约 41% 的籽铁，占 至少部分原因是豆类种子中铁浓度高，并且 大豆种子中的大部分铁都存在于铁蛋白中。 为了 进一步了解种子铁蛋白与种子铁蛋白之间的关系 生物可利用的铁，缺铁和铁充足的实验 建议人类分析：（1）大豆铁蛋白的利用 大豆粉、豆腐或肉汤中的铁；抗坏血酸的作用和 植酸盐的吸收也将被测定以评估 抑制剂和增强剂的影响。 (二)大豆的功效 大豆铁蛋白保留的加工。 如果时间允许的话， 将在杂交中分析铁蛋白启动子和种子铁蛋白 铁蛋白和可溶性铁含量高或低的品种 允许未来开发铁含量更高的大豆 有助于可持续地解决人类缺铁问题。