3 DIMENSIONAL STRUCTURE OF IRE IN FERRITIN MRNA, STUDIED BY NMR SPECTROSCOPY

通过核磁共振波谱研究铁蛋白 mRNA 中 IRE 的三维结构

• 批准号: 6309021
• 负责人: ELIZABETH C THEIL
• 金额: $ 2.74万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2002-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6309021
• 关键词: Chordata; biomedical resource; biotechnology; hematology; liver; nucleic acids; nutrition; nutrition related tag; technology /technique

The SIR provided L-AMinine-guanido- 13C, "N2; 15mg 'C,'N RNA; 1.2g 13C, "N RNA; 292mg 13C, "N RNA; 320mg "C, "N RNA; 560mg The IRE cis element in eukaryotic mRNA is highly conserved and has a characterized specific regulator protein. Our studies of function and structure, using site-directed mutagenesis and cleavage by shape-selective transition metal complexes as structure probes, indicate a folded structure with possibly unusual tertiary features. Our preliminary NMR spectroscopy shows that the IRE sequence (a 30 mer) forms a single conformer in solution which facilitates the structure analysis. The use of 13C- and 15N-enriched ribonucleotides, uniformly labelled or otherwise, are crucial to the NMR study.

SIR提供了l-氨基氨基 - 瓜烷 - 13C，“ N2; 15mg 'c，'n rna; 1.2克 13c，“ N RNA; 292mg 13c，“ N RNA; 320mg “ C，” N RNA； 560mg真核mRNA中的顺式元素高度高 保守并具有特异性调节蛋白。 我们的 使用位置定向的诱变和功能和结构的研究 形状选择性过渡金属复合物作为结构的裂解 探针，表示可能不寻常的第三纪的折叠结构 特征。 我们的初步NMR光谱表明IRE 序列（30 mer）在溶液中形成单个构象体 促进结构分析。 富含13C和15N的使用 核糖核苷酸，均匀标记或以其他方式，对 NMR研究。