BIOSYNTHESIS OF AZETIDINE-2-CARBOXYLIC ACID

该项目的目的是建立生物合成途径，为 氮杂环丁烷-2-羧酸在微生物中的形成 并进一步阐明铁锈菌的立体过程。 从适当标记的前体形成A-2-C。区域和 立体特定的同位素标记底物和产品(A-2-C)将 用来获得有关这种酶的作用机制的信息。这个 酶反应的全立体过程将从总和中阐明。 发生在α-、β-和α-的单个立体化学事件 每种底物氨基酸的伽马碳。 将使用酶和化学方法来合成 区域和立体特异的13C和2H标记底物氨基酸和 A-2-C基板、产品等的配置将是 用~1H-，~2H-和~(13)C-核磁共振、质谱学相结合的方法测定。和Ord 和CD。

The aim of this project is to establish the biosynthetic pathway for the formation of azetidine-2-carboxylic (A-2-C) acid in the microorganism Actinoplanes ferrugineus and further to elucidate the steric course of the formation of A-2-C from the appropriately labeled precursor. Regio- and stereo-specific isotopically labeled substrates and products (A-2-C) will be used to gain information concerning the mechanism of this enzyme. The total steric course of the enzyme reaction will be elucidated from the sum of the individual stereochemical events occurring at the Alpha-, Beta-, and Gamma-carbons of each substrate amino acid. Both enzymatic and chemical methodology will be utilized to synthesize the regio- and stereo-specific 13C- and 2H-labeled substrate amino acids and A-2-C. The configuration of the substrate, products, etc. will be determined using a combination of 1H-, 2H- and 13C-NMR, mass spec. and ORD and CD.