CONTROL OF TRANSCRIPTION TERMINATION IN E. COLI

噬菌体lambda染色体上的转录终止被抑制 通过病毒N蛋白。阻止N作用的宿主突变(Nus，Gron) 也影响细菌染色体上的转录终止。我们发现 NusB突变体在转录核糖体RNA方面有缺陷；我们是 选择更多具有这一缺陷的大肠杆菌突变体。我们设计了一种 新的波长载体来执行这一选择，并验证 在这些突变体中，转录终止受到影响。 其他改变转录终止的大肠杆菌突变已经被 被分离出来作为nusAl表型的抑制因子。一个这样的抑制者 似乎存在于基因“u”中，它编码一个21kd的蛋白质，位于 一组参与信使核糖核酸翻译的基因。我们希望确定 “U”产品的性质，并询问它是否作为 转录和翻译。 噬菌体lambda不在噬菌体溶原性大肠杆菌上繁殖 香港022。这种排除是由于HK022的前噬菌体功能，Nun，它起作用 作为一种新的转录终止因子。修女与NUS因素互动 在Lambda坚果店引发提前终止妊娠。

Transcription termination on the coliphage lambda chromosome is suppressed by the viral N protein. Host mutations which block N action (nus, groN) also affect transcription termination on the bacterial chromosome. We find that nusB mutants are defective in transcribing ribosomal RNA; we are selecting additional E. coli mutants with this defect. We have designed a novel lambda vector to perform this selection, and to verify that transcription termination is affected in these mutants. Other E. coli mutations that alter transcription termination have been isolated as suppressors of the nusAl phenotype. One such suppressor appears to lie in gene "u", which encodes a 21 kd protein and is located in a cluster of genes involved in mRNA translation. We wish to determine the nature of the "u" product, and to ask if it serves as a link between transcription and translation. Bacteriophage lambda does not propagate on E. coli lysogenic for phage HK022. This exclusion is due to a HK022 prophage function, Nun, which acts as a new transcription termination factor. Nun interacts with Nus factors to provoke premature termination at the lambda nut sites.