CONTROL OF TRANSCRIPTION TERMINATION IN E COLI

对噬菌体的研究在生物学领域有着极其重要的意义。 生成关于通用监管机制的信息。 转录终止系统对基因表达的调控 - 抗终止，首先在噬菌体中描述，现在已经在 真核细胞，特别是控制c-myc和HIV基因 表情 细胞因子，病毒蛋白，和特定的网站所需的 抗终止在λ噬菌体系统中被部分理解， 几乎完全未知的噬菌体HKO 22。的差异和 这两个人之间的相似之处，以及相互作用， 他们的监管制度，正在探索。 该方法是 包括遗传和生物化学，并涉及突变体的分离， 调节蛋白的过量生产和纯化， 突变位点的构建。 另一种噬菌体调节系统，阻遏，受噬菌体的控制。 主机函数RpsB（S2）。 对照组为mRNA水平 翻译;该法规的机制将由以下内容确定： 突变体分析和体外翻译系统的建立。

The study of the ten"rate bacteriophage has been exceedingly useful in generating information about universal regulatory mechanisms. Regulation of gene expression by a system of transcription termination - antitermination, first described in phage, has now been seen in eukaryotic cells, in particular the control of c-myc and HIV gene expression. The cellular factors, viral proteins, and specific sites required for antitermination are partially understood in the phage lambda system, and almost completely unknown for phage HKO22. The differences and similarities between these two phages, as well as the interactions betwen their regulatory systems, is being explored. The approach is both genetic and biochemical, and involves the isolation of mutants, the overproduction and purification of regulatory proteins, and the construction of mutant sites. Another phage regulatory system, repression, is subject to control by a host function, RpsB (S2). The control is at the level of mRNA translation; the mehanism of this regulation will be determined by analysis of mutants and establishment of an in vitro translation system.