Super-resolution imaging across the Biosciences

跨生物科学领域的超分辨率成像

• 批准号: BB/X019233/1
• 负责人: Michelle Peckham
• 金额: $ 38.34万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX019233%2F1
• 关键词: Super; resolution; imaging; across; Biosciences

Labelling specific objects in samples such as cells and tissues with a fluorescent dye, and then imaging them using a fluorescent microscope, is a tool that has revolutionised our ability to discover how proteins, DNA, RNA and other cellular structures are organised within cells and tissues. However, until recently, the ability to resolve the fine detail was limited to about 0.25 microns (approximately 300x smaller than the width of a human hair). This is because the amount of fine detail or 'resolution' depends on the wavelength of light and the so-called numerical aperture of the objective lens used to image the sample, two properties that cannot be altered beyond a fixed limit. However, about 20 or so years ago, new approaches to overcome this resolution barrier, collectively known as super-resolution imaging, started to be developed, leading to the award of the Nobel Prize to key developers of this technology (Hell, Moerner and Betzig) in 2014. Super-resolution imaging is rapidly becoming a key tool for researchers to uncover the fine detail of how objects are organised within cells, revealing new detail that we did not previously know existed. In this research, we want to open up a specific type of super-resolution imaging called 'single molecule localisation microscopy' to a wide range of researchers, by implementing a commercial system that is easy to use, multifunctional and can image in much great detail, almost 20x better than we can currently achieve with a standard fluorescence microscope. This new microscope will reveal new insight into protein organisation within cells and tissues across a range of research areas, from cancer to heart disease and blindness.

用荧光染料标记细胞和组织等样品中的特定物体，然后使用荧光显微镜对它们进行成像，这是一种工具，它彻底改变了我们发现蛋白质、DNA、RNA和其他细胞结构在细胞和组织内是如何组织的能力。然而，直到最近，解决精细细节的能力被限制在大约0.25微米（大约比人类头发宽度小300倍）。这是因为精细细节的数量或“分辨率”取决于光的波长和用于对样品成像的物镜的所谓数值孔径，这两个属性不能超过固定的限制。然而，大约20年前，克服这一分辨率障碍的新方法，统称为超分辨率成像，开始被开发出来，导致2014年诺贝尔奖授予这项技术的主要开发者（Hell， Moerner和Betzig）。超分辨率成像正迅速成为研究人员揭示细胞内物体组织细节的关键工具，揭示了我们以前不知道的新细节。在这项研究中，我们希望通过实施一个易于使用，多功能，可以非常详细地成像的商业系统，向广泛的研究人员开放一种称为“单分子定位显微镜”的特定类型的超分辨率成像，几乎比我们目前使用标准荧光显微镜所能达到的效果好20倍。这种新型显微镜将揭示一系列研究领域中细胞和组织内蛋白质组织的新见解，从癌症到心脏病和失明。