A pipeline for high-throughput microbial isolation, sorting, screening and synthetic community assembly

用于高通量微生物分离、分选、筛选和合成群落组装的管道

• 批准号: BB/X01942X/1
• 负责人: James McDonald
• 金额: $ 118.8万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX01942X%2F1
• 关键词: pipeline; throughput; microbial; isolation; sorting

Microorganisms live as complex communities of different species (e.g. of bacteria and fungi) and play key roles in health and disease in plants and animals, support global nutrient cycles, and represent a source of diverse metabolic pathways and enzymes that can be used to address grand societal challenges in medicine, agriculture and industry. Due to the complexity of microbial communities that can contain millions of cells and thousands of individual species of microbe, separating out individual cells to grow and study them as pure cultures in the laboratory, sequence their genome, understand their physiology, or to screen them for new enzymes and discover new microbial compounds (e.g. antibiotics) is a huge challenge. However, this is now made possible by emerging advanced technologies that separate single cells into microdroplets and select or sort droplets for further laboratory analysis. Here, we will combine three pieces of equipment to develop an experimental pipeline that will enable us to separate single microbial cells from a complex community (or population of cells) and encapsulate them in water/oil or gel droplets where millions of droplets can then be sorted and selected for further screening. This approach uses new advanced research technology that is currently not widely available and will significantly increase the speed at which millions of cells/droplets can be analysed. The equipment will be accessible to a broad user community and will support rapid and significant research advances that will drive a step-change in our ability to isolate microbes into pure culture, to study their biology, and discover new enzymes and microbial compounds that can address grand challenges relating to plant and human health, microbial function, enzyme discovery, screening for antimicrobial resistance, discovery of new antimicrobial compounds, waste management and sustainable energy generation.

微生物作为不同物种（例如细菌和真菌）的复杂群落生活，在植物和动物的健康和疾病中发挥关键作用，支持全球营养循环，并代表可用于应对医学，农业和工业重大社会挑战的多种代谢途径和酶的来源。由于微生物群落的复杂性，可以包含数百万个细胞和数千种微生物，分离出单个细胞在实验室中作为纯培养物生长和研究它们，对其基因组进行测序，了解它们的生理学，或筛选它们的新酶并发现新的微生物化合物（例如抗生素）是一个巨大的挑战。然而，现在通过新兴的先进技术将单细胞分离成微滴并选择或分选微滴以进行进一步的实验室分析，这成为可能。在这里，我们将联合收割机三件设备开发一个实验管道，使我们能够从复杂的群落（或细胞群）中分离出单个微生物细胞，并将它们封装在水/油或凝胶液滴中，然后可以对数百万个液滴进行分选和选择以进行进一步筛选。这种方法使用了目前尚未广泛使用的新的先进研究技术，并将显著提高分析数百万细胞/液滴的速度。该设备将提供给广泛的用户社区，并将支持快速和重大的研究进展，这将推动我们将微生物分离到纯培养物中的能力的一步变化，研究它们的生物学，并发现新的酶和微生物化合物，这些酶和微生物化合物可以解决与植物和人类健康，微生物功能，酶发现，抗菌素抗性筛选，发现新的抗菌化合物、废物管理和可持续能源生产。