MOLECULAR ANALYSIS OF STRESS INDUCED GENES IN CARROT

胡萝卜应激诱导基因的分子分析

基本信息

批准号：
3293175
负责人：
J LYNN ZIMMERMAN
金额：
$ 10.87万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE COUNTY
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-12-01 至 1990-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3293175
关键词：
DNA developmental genetics environmental stressor ethylenes gene expression genetic promoter element heat hydroxyproline molecular cloning mutant nucleic acid sequence plant genetics plant growth /development radiotracer stress proteins structural genes vegetables

项目摘要

We propose to analyze the molecular basis for the regulated expression of two important gene sets in higher plants, the heat shock gene family and the cell wall hydroxyproline rich glycoprotein (HPRGP) gene. Both gene sets are highly inducible, at both the mRNA and protein levels, by important physiological stresses, namely heat shock and ethylene. Furthermore, we now have evidence which indicates that the HPRGP gene is independently regulated by both ethylene and heat shock, and there is reason to believe that subsets of the heat shock gene family will exhibit independent developmental regulation as well in our system, carrot. Thus, we have in these two gene sets the potential to study multiple regulatory regions governing single structural genes which may serve vital physiological roles both during normal development and in response to stress. We propose to delimit those DNA sequences of each gene set responsible for these forms of regulation by modifying and/or deleting potentially important regions and assaying their ability to function in a regulated fashion after transformation back into carrot cells. This type of detailed molecular analysis is an essential step toward utilizing these highly inducible "promoter" sequences to direct the controlled expression of potentially useful agronomic traits, and represents a fundamental beginning toward crop improvement through biotechnology.

我们建议分析调节表达的分子基础高等植物中的两个重要基因集，热休克基因家族和细胞壁羟蛋白富糖蛋白（HPRGP）基因。两个基因在mRNA和蛋白质水平上，集合都是高度诱导的，重要的生理应力，即热休克和乙烯。此外，我们现在有证据表明HPRGP基因是由乙烯和热冲击独立调节，并且有有理由相信热冲击基因家族的子集将表现出来我们系统中的独立发育法规，胡萝卜。因此，我们在这两个基因中有研究多重调节的潜力控制单个结构基因的区域可能有至关重要在正常发育期间和对压力。我们建议划定每个基因集的DNA序列，负责通过修改和/或删除这些形式的调节形式重要区域并分析其在受监管中运作的能力转化回到胡萝卜细胞后的时尚。这种详细的分子分析是利用这些高度利用这些的重要步骤可诱导的“启动子”序列，以指导潜在有用的农艺特征，代表一个基本的开始通过生物技术改善作物。