ROLE OF PLACENTAL LACTOGENS IN PREGNANCY

胎盘泌乳素在妊娠中的作用

• 批准号: 3312911
• 负责人: FRANK J. TALAMANTES
• 金额: $ 19.58万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3312911
• 关键词: catecholamines; centrifugation; gel electrophoresis; gel filtration chromatography; gestational age; growth /development; hormone biosynthesis; hormone regulation /control mechanism; mammary gland; ovary; placental hormones; progesterone; prolactin; radioimmunoassay; radiotracer; somatotropin; testosterone; ultrafiltration

The mouse conceptus produces two lactogenic hormones, placental lactogen (PL) and midpregnancy lactogen. The objectives of this proposal are (1) to study the regulation of secretion of PL and (2) to examine the biological activities of PL and midpregnancy lactogen and two other lactogenic hormones, prolactin (PRL) and growth hormone (GH), in several target tissues. Regulation of PL secretion by the pituitary, ovaries, fetus and decidua and by growth factors will be studied. In addition, the influence of genetic factors on mPL secretion will be examined. A system for primary culture of placental trophoblast giant cells in serum-free medium will be established and the effects of various hormones and growth factors on mPL secretion and on cell growth will be examined. PL, midpregnancy lactogen, PRL and GH are all present during pregnancy in the mouse. The effects of each of these hormones on target tissues will be studied to assess the relative potencies of the hormones in each tissue and to determine whether tissues at different stages of pregnancy respond preferentially to some of the hormones. The effects of these hormones on mammary gland casein, Alpha-lactalbumin and fatty acid synthesis will be determined. The ability of these hormones to stimulate mammary gland epithelial cell growth and to bind to mammary gland receptors will be evaluated. The effects of these hormones on somatomedin produced by maternal and fetal liver will be studied. The ability of these hormones to stimulate fetal growth will be assessed. The studies proposed in this application will be carried out with hormones purified from the mouse to study the endocrinology or pregnancy in the mouse. Thus the results of these studies will not be subject to problems of interpreting data collected from heterologous systems. PL is present in humans and serum PL levels have been used as one index of fetal well-being. The studies in this proposal will provide information about the physiology of this hormone and the interaction of this hormone with other hormones with similar activities during pregnancy in an animal model. The information obtained from studies in this animal model may prove relevant to the human.

小鼠胚胎产生两种催乳素，即胎盘催乳素 (Pl)和孕中期催乳素。这项建议的目标是(1) 研究磷脂酶的分泌规律和(2)检测其生物学特性 孕中期催乳素和另外两种催乳素的活性 多个靶点的激素，催乳素(PRL)和生长激素(GH) 纸巾。 垂体、卵巢、胎儿和蜕膜对PL分泌的调节 按生长因子进行研究。此外，遗传因素的影响 我们将研究影响MPL分泌的因素。 胎盘滋养层巨细胞原代培养体系的建立 将建立无血清培养基及各种激素的影响 并将检测生长因子对MPL分泌和细胞生长的影响。 孕期存在催乳素、孕中期催乳素、催乳素和生长激素。 那只老鼠。每种激素对靶组织的影响将是 研究评估每个组织中激素的相对效力，并 以确定怀孕不同阶段的组织是否会对 优先于某些荷尔蒙。这些激素对人的影响 乳腺酪蛋白、α-乳白蛋白和脂肪酸的合成 下定决心。这些激素刺激乳腺的能力 上皮细胞生长并与乳腺受体结合将是 已评估。这些激素对沙门氏菌产生生长抑素的影响 将对母体和胎儿肝脏进行研究。这些荷尔蒙的能力 将对刺激胎儿生长进行评估。 本申请中提出的研究将使用激素进行。 从小鼠中提纯，用于研究小鼠的内分泌学或妊娠 老鼠。因此，这些研究的结果不会有问题。 解释从不同系统收集的数据。 PL存在于人类中，血清PL水平已被用作 胎儿健康。本提案中的研究将提供信息 关于这种激素的生理和这种激素的相互作用 在动物怀孕期间与其他具有类似活动的激素 模特。从该动物模型的研究中获得的信息可能 证明与人类有关。