STUDIES ON MAMMALIAN OOCYTES (AND SPERMATOCYTES)

哺乳动物卵母细胞（和精母细胞）的研究

• 批准号: 3315883
• 负责人: GEORGIANA M JAGIELLO
• 金额: $ 28.46万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3315883
• 关键词: DNA methylation; cell differentiation; cell nucleus; egg /ovum; electron microscopy; gene expression; hamsters; human tissue; hypothalamic hormones; laboratory mouse; meiosis; methylation; microscopy; mitochondrial DNA; natural gene amplification; oogenesis; sex chromosomes; sperm; spermatogenesis

The investigations in this application propose to study the meiotic characteristics of the adult male and female Turkish hamster during prehibernation, hibernation and recrudescence in a short photoperiod/cold environment. Analysis of key steps in meiotic progression will be carried out simultaneous with relevant endocrine and thermal determinations. In the male, it is proposed to study the kinetics of meiotic stages, and analyze the duration of preleptotene DNA synthesis and replicon characteristics. Analysis of pachytene spermatocyte chromomere maps, synaptonemal complexes and Z-DNA sites is proposed. Recombination as assessed by chiasma will be done in diplotene spermatocytes with identification of individual bivalents by chromomere maps. Extraction and assay of recombination proteins will be made as one index of the normalcy of underlying genetic recombination. Chromosomal complements at metaphase I and II spermatocytes provide measures of fidelity of earlier stages and induced aberrations. The focus of investigation under defined photoperiod/temperature conditions in female Turkish hamster oogenesis will be to identify aneuploidy and structural abnormalities in oocytes matured in vitro and in vivo. It is also proposed to assess fertilized oocytes for transmissible abnormalities which may have been induced. The capability for studying the recrudescence of spermatogenesis and the later stages of oocyte maturation in a physiological condition using reduced temperature, a known perturbator of meiosis, as a probe presents a unique system for analyzing essential stages of germ cell development in mammals and correlating these with endocrine factors. The feasibility of the such studies has been demonstrated in a preliminary study with male Mesocricetus auratus.

在这方面的研究建议对减数分裂进行研究