STUDIES ON MAMMALIAN OOCYTES (AND SPERMATOCYTES)

哺乳动物卵母细胞（和精母细胞）的研究

• 批准号: 2197725
• 负责人: GEORGIANA M JAGIELLO
• 金额: $ 28.24万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2197725
• 关键词: DNA methylation; DNA replication; Z DNA; cell differentiation; cell nucleus; egg /ovum; electron microscopy; female; gene expression; hamsters; human tissue; hypothalamic hormones; laboratory mouse; meiosis; methylation; microscopy; mitochondrial DNA; natural gene amplification; oogenesis; replicon; sex chromosomes; sperm; spermatogenesis

The investigations in this application propose to study the meiotic characteristics of the adult male and female Turkish hamster during prehibernation, hibernation and recrudescence in a short photoperiod/cold environment. Analysis of key steps in meiotic progression will be carried out simultaneous with relevant endocrine and thermal determinations. In the male, it is proposed to study the kinetics of meiotic stages, and analyze the duration of preleptotene DNA synthesis and replicon characteristics. Analysis of pachytene spermatocyte chromomere maps, synaptonemal complexes and Z-DNA sites is proposed. Recombination as assessed by chiasma will be done in diplotene spermatocytes with identification of individual bivalents by chromomere maps. Extraction and assay of recombination proteins will be made as one index of the normalcy of underlying genetic recombination. Chromosomal complements at metaphase I and II spermatocytes provide measures of fidelity of earlier stages and induced aberrations. The focus of investigation under defined photoperiod/temperature conditions in female Turkish hamster oogenesis will be to identify aneuploidy and structural abnormalities in oocytes matured in vitro and in vivo. It is also proposed to assess fertilized oocytes for transmissible abnormalities which may have been induced. The capability for studying the recrudescence of spermatogenesis and the later stages of oocyte maturation in a physiological condition using reduced temperature, a known perturbator of meiosis, as a probe presents a unique system for analyzing essential stages of germ cell development in mammals and correlating these with endocrine factors. The feasibility of the such studies has been demonstrated in a preliminary study with male Mesocricetus auratus.

本申请中的研究旨在研究减数分裂 成年雄性和雌性土耳其仓鼠的特征 短暂的冬眠前、冬眠和复苏 光周期/寒冷环境。 减数分裂关键步骤分析 进展将与相关的同时进行 内分泌和热测定。 在男性中， 建议研究减数分裂阶段的动力学，并分析 前细线 DNA 合成和复制子的持续时间 特征。 粗线期精母细胞染色体分析 提出了图谱、联会复合体和 Z-DNA 位点。 通过交叉评估的重组将在双线期中进行 精母细胞通过鉴定个体二价体 染色体图。 重组蛋白的提取和测定 将作为潜在遗传正常性的指标之一 重组。 中期 I 和 II 的染色体补体 精母细胞提供早期阶段保真度的测量， 诱发像差。 规定光周期/温度下的研究重点 雌性土耳其仓鼠卵子发生的条件将是确定 体外成熟卵母细胞的非整倍性和结构异常 和体内。 还建议评估受精卵母细胞的 可能已诱发的传染性异常。 研究精子发生复发的能力 以及卵母细胞成熟的后期阶段 使用降低温度的条件，已知的扰动器 减数分裂作为探针提供了一种独特的分析系统 哺乳动物生殖细胞发育的重要阶段 将这些与内分泌因素相关联。 的可行性 一项针对男性的初步研究已经证明了此类研究 金环仓鼠。