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STUDIES ON MAMMALIAN OOCYTES (AND SPERMATOCYTES)

哺乳动物卵母细胞（和精母细胞）的研究

基本信息

批准号：
2197726
负责人：
GEORGIANA M JAGIELLO
金额：
$ 5.46万
依托单位：
COLUMBIA UNIV NEW YORK MORNINGSIDE
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-04-01 至 1995-07-31
项目状态：
已结题

项目摘要

The investigations in this application propose to study the meiotic characteristics of the adult male and female Turkish hamster during prehibernation, hibernation and recrudescence in a short photoperiod/cold environment. Analysis of key steps in meiotic progression will be carried out simultaneous with relevant endocrine and thermal determinations. In the male, it is proposed to study the kinetics of meiotic stages, and analyze the duration of preleptotene DNA synthesis and replicon characteristics. Analysis of pachytene spermatocyte chromomere maps, synaptonemal complexes and Z-DNA sites is proposed. Recombination as assessed by chiasma will be done in diplotene spermatocytes with identification of individual bivalents by chromomere maps. Extraction and assay of recombination proteins will be made as one index of the normalcy of underlying genetic recombination. Chromosomal complements at metaphase I and II spermatocytes provide measures of fidelity of earlier stages and induced aberrations. The focus of investigation under defined photoperiod/temperature conditions in female Turkish hamster oogenesis will be to identify aneuploidy and structural abnormalities in oocytes matured in vitro and in vivo. It is also proposed to assess fertilized oocytes for transmissible abnormalities which may have been induced. The capability for studying the recrudescence of spermatogenesis and the later stages of oocyte maturation in a physiological condition using reduced temperature, a known perturbator of meiosis, as a probe presents a unique system for analyzing essential stages of germ cell development in mammals and correlating these with endocrine factors. The feasibility of the such studies has been demonstrated in a preliminary study with male Mesocricetus auratus.

这一应用中的研究建议研究减数分裂。成年雄性和雌性土耳其仓鼠在发育过程中的特征冬眠前、冬眠和复发光周期/寒冷环境。减数分裂的关键步骤分析进展将与相关部门同时进行内分泌和体温测定。在雄性中，建议对减数分裂阶段的动力学进行研究，并分析前纤毛虫DNA合成和复制子的持续时间特点。粗线期精母细胞染色单体的分析提出了MAP、联会复合体和Z-DNA位点。交叉评估的重组将在双线期进行。精母细胞对单个二价体的鉴定色度图。重组蛋白的提取及检测将作为潜在遗传基因正常性的一个指标重组。中期I和II的染色体补体精母细胞提供了早期阶段的保真度和诱发像差。确定的光周期/温度下的研究重点雌性土耳其仓鼠的卵子发生条件将被鉴定体外成熟卵母细胞的非整倍体和结构异常在活体内。还建议对受精卵母细胞进行评估可能已被诱发的可传播异常。精子发生复发的研究能力而卵母细胞成熟的后期在生理上使用降温的条件，一个已知的微扰子减数分裂，作为一种探针，提供了一种独特的分析系统哺乳动物生殖细胞发育的基本阶段和将这些与内分泌因素联系起来。这项计划的可行性这种研究已经在一项针对男性的初步研究中得到了证实。金黄中蜂属。