STUDIES OF MAMMALIAN OOCYTES

哺乳动物卵母细胞的研究

• 批准号: 3315881
• 负责人: GEORGIANA M JAGIELLO
• 金额: $ 19.56万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3315881
• 关键词: DNA methylation; cell differentiation; cell nucleus; egg /ovum; electron microscopy; gene expression; human tissue; meiosis; methylation; microscopy; mitochondrial DNA; natural gene amplification; oogenesis; sex chromosomes; sperm; spermatogenesis

The work in these laboratories has for some years focused on mammalian meiosis, with particular emphasis on oogenesis. We have been concerned with the cytologic, biochemical, ultrastructural and hormonal aspects of this process in mammals, particularly human. This attention stems from the fundamental interest in the causes of abnormal progeny in the human, particularly the progeny who result from numerical or structural abnormalities in the germ cell genome. The present application takes a new direction built upon the body of data and hypotheses which hane resulted from this interest. The application process to examine (1) the characteristics of fetal mouse oogonia proceeding toward meiosis (2) chemical and conformational modifications of nuclei and mitochondria of oogeneis and spermatogenesis and (3) the model that distortion of pairing of homologues by microtubule inhibiting agents affects recombination. These studies bring together the biology of germ cell development and the simultaneous changes in the nucleus.

这些实验室的工作多年来一直集中在哺乳动物身上。 减数分裂，特别强调卵子发生。 我们一直担心 细胞学、生物化学、超微结构和激素方面， 这个过程在哺乳动物，特别是人类。 这种关注源于 对人类异常后代原因的根本兴趣， 特别是由数量或结构决定的后代 生殖细胞基因组的异常 本申请采用新的实施方式。 方向建立在数据和假设的基础上， 从这个兴趣。 申请过程审查（1） 小鼠胚胎卵原细胞减数分裂的特征（2） 细胞核和线粒体的化学和构象修饰 卵子发生与精子发生的关系;（3）配对畸变模型 同源物的微管抑制剂影响重组。 这些研究将生殖细胞发育的生物学和 核的同时变化。