IMMUNOREGULATION IN PREGNANCY

怀孕期间的免疫调节

• 批准号: 3314682
• 负责人: Gary W. Wood
• 金额: $ 11.14万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3314682
• 关键词: cellular immunity; flow cytometry; gene expression; granulocyte; histocompatibility antigens; immunofluorescence technique; immunoregulation; immunosuppression; lymphocyte; macrophage; mixed lymphocyte reaction test; placenta; placental transfer; pregnancy; pregnancy immunology; prostaglandins; tissue /cell culture; uterus; vitelline membrane

The mammalian fetus in an outbred population invariably expresses antigens, some of them histocompatability antigens which are derived from both parents. If those antigens were expressed by allografts of other tissue, the grafts would be rejected, yet the fetus develops with few complications within the uterine environment. Obviously this is an immunologic enigma. A general theory has evolved which suggests that one reason for survival of the fetal allograft is the development of local immunoregulation. The rationale for the present proposal is that tissues surrounding the fetus, the uterus, placenta and yolk sac, contain suppressor cells which prevent rejection of fetal tissue. The research described herein is designed to localize, identify and quantify immunologic cells (lymphocytes, macrophages and granulocytes) within tissue that surrounds the fetus, i.e., the uterus, placenta and yolk sac. It is then designed to determine the degree of suppressor cell activity for T-lymphocyte mediated cell function, determine the nature of the suppressor cells and begin to understand by what mechanism the suppressor cells act to modulate lymphocyte function. Finally experiments will be performed to evaluate the effect on pregnancy of agents which block the activity of the putative suppressor cells. These series of experiments should allow us to determine 1) if immunoregulation occurs and is important in promoting fetal survival and 2) what cells are responsible for immunoregulation.

远系繁殖的哺乳动物胎儿总是表达抗原， 它们中的一些是来源于两者的组织相容性抗原， 父母 如果这些抗原在其他组织的同种异体移植物中表达， 移植物会被排斥，但胎儿的发育几乎没有并发症。 在子宫环境中。 显然，这是一个免疫学之谜。 一个普遍的理论已经发展出来，它表明，一个生存的原因， 胎儿移植是局部免疫调节的发展。 的 本建议的基本原理是胎儿周围的组织， 子宫、胎盘和卵黄囊含有抑制细胞， 胎儿组织的排斥反应 本研究旨在 定位、鉴定和定量免疫细胞（淋巴细胞、巨噬细胞 和粒细胞），即，子宫， 胎盘和卵黄囊。 然后，它被设计为确定 T淋巴细胞介导的细胞功能抑制细胞活性，测定 抑制细胞的性质，并开始了解 抑制细胞调节淋巴细胞功能的机制。 最后将进行实验以评估对妊娠的影响 阻断假定的抑制细胞活性的药物。 这些 一系列的实验应该使我们能够确定1）如果免疫调节 发生并在促进胎儿存活方面很重要，2）什么细胞是 负责免疫调节。