REGULATION OF XENOPUS HISTONE GENE TRANSCRIPTION

总体目标是研究转录调控的作用。 胚胎发育过程中基因表达调控的序列 和差异化。具体目标是确定和 鉴定与组蛋白相关的转录调控序列 非洲爪哇的基因及其可能的鉴定和特征 与调控序列相互作用的蛋白质因子。传统型 体外核酸操作方法将用于构建突变体 含有缺失、插入和重排的组蛋白基因。 操纵的基因将被显微注射到非洲爪哇卵母细胞和他们的 表达将通过放射性标记的RNA的电泳法进行研究。活体内 并将建立推测的调控序列结合的体外检测方法 蛋白质。这些分析将被用来研究蛋白质之间的相互作用。 具有特定的RNA序列，并对所涉及的蛋白质进行表征。

The general objective is to study the roles of transcriptional regulatory sequences in the control of gene expression during embryonic development and differentiation. The specific objectives are to identify and characterize transcriptional regulatory sequences associated with histone genes of Xenopus laevis and to identify and characterize the putative protein factors that interact with the regulatory sequences. Conventional in vitro nucleic acid manipulation methods will be used to construct mutant histone genes containing deletions, insertions, and rearrangements. Manipulated genes will be microinjected into Xenopus oocytes and their expression will be studied by electrophoresis of radiolabeled RNA. In vivo and in vitro assays will be set up for putative regulatory sequence-binding proteins. These assays will be used to examine the interaction of proteins with specific RNA sequences and to characterize the proteins involved.