FUNCTION OF MYOGENIC FACTORS IN EARLY DEVELOPMENT

几种不同转录调控因子的分离 多能干细胞的肌源性决定所需的 最近报道的。该序列的分子克隆及鉴定 在结构和功能上相关的调控基因 赤霉菌末端分化相关事件的分子分析 肌肉细胞。在脊椎动物中，肌源性谱系起源于中胚层。 在原肠胚处从外胚层和内胚层分离。 胚胎发育阶段。人们对生殖系知之甚少 脊椎动物胚胎在分子水平上的规范，但它是 合理预测参与中胚层发育的调节因子 胚胎发育早期的发育和肌源性决定可能是 与那些参与肌肉细胞后期的基因有关 差异化。我们最近分离到了几个与MyoD1相关的cDNA 在体节形成和肌肉形成之前存在于非洲爪哇胚胎中的 细胞分化；这些克隆被命名为XLMF，用于非洲爪哇 生肌因子。这项研究的目的是进一步描述 这些cdna序列，以检测它们在信号传递规范中的作用。 在发育中的青蛙胚胎中的肌源性细胞谱系及其调控 它们的表达，并确定其他相关基因是否 在发育早期表现出来的。

The isolation of several distinct transcriptional regulatory factors required for myogenic determination of pluripotent stem cells has been recently reported. The molecular cloning and characterization of this set of structurally and functionally related regulatory genes allows a molecular analysis of events involved in the terminal differentiation of muscle cells. in vertebrates, the myo genic lineage derives from mesoderm which becomes segregated from the ectoderm and endoderm at the gastrula stage of embryonic development. Little is known about germ line specification in vertebrate embryos at the molecular level, but it is reasonable to anticipate that regulatory factors participating in mesoderm development and myogenic determination early in embryogenesis might be related to those involved in the later stages of muscle cell differentiation. We have recently isolated several MyoD1-related cDNAs that are present in Xenopus embryos prior to somite formation and muscle cell differentiation; these clones were designated XLMF, for Xenopus laevis myogenic factor. The purpose of this study is to further characterize these cDNA sequences, to examine their role in signaling the specification of myogenic cell lineages in developing frog embryos and the regulation of their expression, and to determine whether additional related genes are expressed early in development.