REGULATION OF XENOPUS HISTONE GENE TRANSCRIPTION

总的目标是研究转录调控因子在细胞内的作用。 胚胎发育过程中控制基因表达的序列 和差异化。 具体目标是确定和 表征与组蛋白相关的转录调控序列 非洲爪蟾的基因，并确定和表征推定的 与调节序列相互作用的蛋白质因子。 常规 体外核酸操作方法将用于构建突变体 含有缺失、插入和重排的组蛋白基因。 将操纵的基因显微注射到非洲爪蟾卵母细胞中， 将通过放射性标记的RNA的电泳来研究表达。 体内 并将建立体外试验，用于推定的调节序列结合 proteins. 这些分析将用于检查蛋白质的相互作用 与特定的RNA序列，并表征相关的蛋白质。

The general objective is to study the roles of transcriptional regulatory sequences in the control of gene expression during embryonic development and differentiation. The specific objectives are to identify and characterize transcriptional regulatory sequences associated with histone genes of Xenopus laevis and to identify and characterize the putative protein factors that interact with the regulatory sequences. Conventional in vitro nucleic acid manipulation methods will be used to construct mutant histone genes containing deletions, insertions, and rearrangements. Manipulated genes will be microinjected into Xenopus oocytes and their expression will be studied by electrophoresis of radiolabeled RNA. In vivo and in vitro assays will be set up for putative regulatory sequence-binding proteins. These assays will be used to examine the interaction of proteins with specific RNA sequences and to characterize the proteins involved.