FUNCTION OF MYOGENIC FACTORS IN EARLY DEVELOPMENT

几种不同转录调控因子的分离 多能干细胞的成肌决定所需的 最近报道。 这一组的分子克隆和表征 结构和功能相关的调控基因的存在， 分子分析的事件参与终末分化的 肌肉细胞 在脊椎动物中，生肌谱系起源于中胚层， 在原肠胚处与外胚层和内胚层分离 胚胎发育的阶段。 对生殖细胞系知之甚少 在分子水平上对脊椎动物胚胎的规格，但它是 合理预期参与中胚层的调节因子 胚胎发育早期的发育和肌形成决定可能是 与那些参与肌肉细胞后期阶段的细胞有关， 分化 我们最近分离了几个MyoD 1相关的cDNA 在体节形成和肌肉形成之前存在于非洲爪蟾胚胎中 细胞分化;这些克隆被命名为XLMF，用于非洲爪蟾 生肌因子 本研究的目的是进一步表征 这些cDNA序列，以检查它们在信号的作用，规范 肌原细胞谱系在发育中的青蛙胚胎和调控 它们的表达，并确定是否有其他相关基因， 在发育早期就表达了。

The isolation of several distinct transcriptional regulatory factors required for myogenic determination of pluripotent stem cells has been recently reported. The molecular cloning and characterization of this set of structurally and functionally related regulatory genes allows a molecular analysis of events involved in the terminal differentiation of muscle cells. in vertebrates, the myo genic lineage derives from mesoderm which becomes segregated from the ectoderm and endoderm at the gastrula stage of embryonic development. Little is known about germ line specification in vertebrate embryos at the molecular level, but it is reasonable to anticipate that regulatory factors participating in mesoderm development and myogenic determination early in embryogenesis might be related to those involved in the later stages of muscle cell differentiation. We have recently isolated several MyoD1-related cDNAs that are present in Xenopus embryos prior to somite formation and muscle cell differentiation; these clones were designated XLMF, for Xenopus laevis myogenic factor. The purpose of this study is to further characterize these cDNA sequences, to examine their role in signaling the specification of myogenic cell lineages in developing frog embryos and the regulation of their expression, and to determine whether additional related genes are expressed early in development.