MATERNAL GUT/MAMMARY GLAND INTERFACE IN LACTATION

哺乳期母体肠道/乳腺界面

• 批准号: 3331987
• 负责人: JARED M DIAMOND
• 金额: $ 24.11万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-06 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3331987
• 关键词: aminoacid transport; bioenergetics; breast surgery; brush border membrane; caloric dietary content; developmental nutrition; digestion; fat body; gastrointestinal absorption /transport; gastrointestinal nutrient absorption; glucose transport; hydrolase; intestine surgery; laboratory mouse; lactation; mammary gland; milk; nutrient bioavailability; nutrient intake activity; nutrition related tag; small intestines

The long-term goal is to understand how a lactating mother's gut meets the mother's increased requirement for nutrients to be exported into milk. The driving motivations are the physiological and clinical significance of lactation, and the advantages of the gut/mammary gland interface as a model for attacking broad questions of quantitative integration within the body. The specific aims, utilizing mouse models, include the following: 1. To identify the ceiling value of dietary nutrient intake during lactation: is it limited to the caloric equivalent of about seven times the basal metabolic rate? 2. To identify the proximate factors limiting lactational nutrient output. Candidate factors include the dietary supply of certain nutrients, mammary milk- producing capacity, intestinal digestive capacity, or the latter two factors matched to each other. 3. To test how physiological capacities are matched to each other and to demand during lactation. Capacities may impose bottlenecks on performance or may exceed demand by some reserve capacity, while series capacities may not to matched to each other. The research design includes experimentally pushing the energy burden of lactation towards a limit by expanding litter size, extending the duration of lactation, or combining the burden of lactation with the burden of increased heat production as low ambient temperatures; varying intestinal digestive capacity surgically, by partial resection of the small intestine; and varying mammary gland secretory capacity surgically, by selective ablation. Activities and capacities of intestinal brush- border and basolateral nutrient transporters and brush-border hydrolases will be measured for comparison with dietary intakes of their substrates.

长期目标是了解哺乳期母亲的肠道如何满足 母亲对营养物质输出的需求增加 牛奶。 驱动动机是生理和临床 哺乳的重要性以及肠道/乳腺的优势 接口作为解决广泛的定量问题的模型 融入体内。 具体目标，利用小鼠模型， 包括以下内容： 1. 确定膳食上限值 哺乳期的营养摄入：是否限于热量当量 大约是基础代谢率的七倍？ 2. 识别 限制泌乳期营养输出的直接因素。 候选人 因素包括膳食中某些营养素的供应、母乳 生产能力、肠道消化能力或后两者 因素相互匹配。 3. 测试生理能力 彼此匹配并满足哺乳期间的需求。 容量可能 造成性能瓶颈或可能超出某些储备的需求 容量，而串联容量可能不匹配。 这 研究设计包括通过实验来减轻能源负担 通过扩大产仔数、延长产仔数，使泌乳量接近极限 哺乳期的持续时间，或将哺乳的负担与 由于环境温度低，产热增加的负担；变化的 通过手术部分切除肠道消化能力 小肠；以及通过手术改变乳腺分泌能力， 通过选择性消融。 肠刷的活动和能力- 边界和基底外侧营养转运蛋白和刷状缘水解酶 将对其底物的膳食摄入量进行测量以进行比较。