ANIMAL MODELS OF EXTREME HYPERPHAGIA AND HIGH METABOLISM

描述：长期目标是开发和利用一种新的动物 研究高吞噬和高代谢率的模型。如下例所示 鱿鱼轴突在我们理解的发展中所起的作用 可兴奋膜，最容易在生物医学研究中取得快速进展 通过研究一个物种中表现出该现象的现象来实现 一种极端的程度，或者以实验上方便的形式。这个项目寻求 相当于胃肠病学领域的鱿鱼轴突， 营养和新陈代谢。 人类和其他哺乳动物表现出许多重要的调控反应 食物进入肠道。尽管如此，这些反应在以下方面是温和的 在通常的实验室哺乳动物中很难进行研究 模型，例如老鼠，因为人类和大多数其他哺乳动物的肠道 适应于经常吃少量食物，很少空闲或休息。相对的 相反，在一些蛇身上观察到了更容易研究的巨大反应 适应不频繁的丰盛食物的物种。 因此，这项提案的具体目标是利用这样一个物种，即 缅甸蟒蛇，作为吞噬和高代谢率的典范。这个 第一个目标是建立实验条件(进餐数量和时间 摄食后)当蟒蛇的调节反应最大时。剩下的 在这些情况下，目标将是识别鲁米诺、荷尔蒙和 其他潜在的胃肠道反应的信号，以识别 肠道调节的分子机制和信号 刷状边缘葡萄糖转运蛋白，并获得最大量的组织 用于分离和测序的蟒蛇多肽激素。

DESCRIPTION: The long term goal is to develop and exploit a new animal model for studying hyperphagia and high metabolic rate. As exemplified by the role of the squid axon in the development of our understanding of excitable membranes, rapid progress in biomedical research is most easily achieved by studying a phenomenon in a species exhibiting that phenomenon to an extreme degree, or in experimentally convenient form. This project seeks the equivalent of a squid axon for the fields of gastroenterology, nutrition, and metabolism. Humans and other mammals exhibit many important regulatory responses to the arrival of food in the gut. Nevertheless, these responses are modest in magnitude and can be difficult to study in the usual laboratory mammal models such as rats, because the guts of humans and most other mammals are adapted to frequent small meals and are rarely empty or at rest. Relatively enormous responses, much easier to study, are instead observed in some snake species adapted to infrequent enormous meals. Hence the specific aims of this proposal are to utilize such a species, the Burmese python, as a model of hyperphagia and high metabolic rates. The first aim is to establish the experimental conditions (meal size and time after feeding) when python regulatory responses are largest. The remaining aims will be, under those conditions, to identify luminal, hormonal and other signals underlying gastrointestinal responses to feeding, to identify molecular mechanisms and signals for regulation of the intestinal brush-border glucose transporter, and to obtain tissues with maximal amounts of python peptide hormones for isolation and sequencing.