ANIMAL MODELS OF EXTREME HYPERPHAGIA AND HIGH METABOLISM

长期目标是开发和利用一种新的动物 用于研究摄食过多和高代谢率的模型。 例证 鱿鱼轴突在我们理解发展中的作用 生物医学研究的快速进展最容易 通过研究一个物种中的一种现象来实现， 一个极端的程度，或在实验方便的形式。 项目的目标是 相当于肠胃病学领域的鱿鱼轴突， 营养和新陈代谢。 人类和其他哺乳动物对这些反应表现出许多重要的调节反应。 食物进入肠道。 然而，这些反应是温和的， 在通常的实验室哺乳动物中难以研究 因为人类和大多数其他哺乳动物的肠道 适应频繁的小餐，很少空着或休息。 相对 相反，在一些蛇身上观察到了巨大的反应， 适应于不经常大量进食的物种。 因此，这项建议的具体目标是利用这样一个物种， 缅甸蟒蛇，作为一个模型的暴食症和高代谢率。 的 第一个目标是建立实验条件（膳食量和时间 喂食后），此时蟒蛇的调节反应最大。 其余 目标将是，在这些条件下，以确定管腔，激素和 胃肠道对进食的反应的其他信号， 调节肠道的分子机制和信号 刷状缘葡萄糖转运蛋白，并获得具有最大量的组织 用于分离和测序。

DESCRIPTION: The long term goal is to develop and exploit a new animal model for studying hyperphagia and high metabolic rate. As exemplified by the role of the squid axon in the development of our understanding of excitable membranes, rapid progress in biomedical research is most easily achieved by studying a phenomenon in a species exhibiting that phenomenon to an extreme degree, or in experimentally convenient form. This project seeks the equivalent of a squid axon for the fields of gastroenterology, nutrition, and metabolism. Humans and other mammals exhibit many important regulatory responses to the arrival of food in the gut. Nevertheless, these responses are modest in magnitude and can be difficult to study in the usual laboratory mammal models such as rats, because the guts of humans and most other mammals are adapted to frequent small meals and are rarely empty or at rest. Relatively enormous responses, much easier to study, are instead observed in some snake species adapted to infrequent enormous meals. Hence the specific aims of this proposal are to utilize such a species, the Burmese python, as a model of hyperphagia and high metabolic rates. The first aim is to establish the experimental conditions (meal size and time after feeding) when python regulatory responses are largest. The remaining aims will be, under those conditions, to identify luminal, hormonal and other signals underlying gastrointestinal responses to feeding, to identify molecular mechanisms and signals for regulation of the intestinal brush-border glucose transporter, and to obtain tissues with maximal amounts of python peptide hormones for isolation and sequencing.