CYTOMEGALOVIRUS PNEUMONITIS: THE ROLE OF TNF A AND B
CYTOMEGALOVIRUS PNEUMONITIS: THE ROLE OF TNF A AND B
批准号:
2217667
负责人:
John D Shanley
金额:
$23.26万
依托单位国家:
美国
项目类别:
财政年份:
1986
资助国家:
美国
项目状态:
已结题
起止时间:
1986-04-01 至 1994-11-30
关键词:
Betaherpesvirinae Herpesviridae disease Pneumocystis pneumonia cytokine disease /disorder model gene expression graft versus host disease host organism interaction immunochemistry immunopathology laboratory mouse lung lung injury nucleic acid hybridization temperature sensitive mutant tumor necrosis factor alpha tumor necrosis factor beta viral pneumonia virus antigen virus cytopathogenic effect virus replication
中文摘要
描述:(改编自申请人的摘要。)虽然严重的CMV
感染可累及多个宿主组织,肺部经常
与致命性巨细胞病毒感染有关。在这些实验中,几只小鼠
将利用CMV感染的模型来探索细胞因子,
肿瘤坏死因子-a和淋巴毒素(TNF-b)作为组织损伤的介质。的作用
亚致死和致死病毒后急性巨细胞病毒感染中的肿瘤坏死因子-a和肿瘤坏死因子-b
我们将对挑战进行审查。使用急性巨细胞病毒感染的模型,
致死性和亚致死性病毒在组织中表达的肿瘤坏死因子数量
比较外源性肿瘤坏死因子对急性巨细胞病毒感染的影响
将对感染情况进行监测。抗体被动转移的效果
将确定肿瘤坏死因子对病毒复制的影响。探索肿瘤坏死因子的作用
在肺损伤中,肺孢子虫相关的MCMV和
将使用移植物抗宿主病,比较在
巨细胞病毒肺部感染和肺孢子虫。肺细胞产生肿瘤坏死因子
将对肺孢子虫进行检查,并将确定是否
产生是抗原特异性的或MHC受限的。被动的影响
抗肿瘤坏死因子抗体和淋巴毒素在巨细胞病毒肺孢子虫上的转移也将
要下定决心。该项目的具体目标是:确定
肿瘤坏死因子-α和淋巴毒素在急性巨细胞病毒感染中的作用
肿瘤坏死因子-α和淋巴毒素在肺孢子虫病发病机制中的作用
与巨细胞病毒联合感染和生殖器疱疹相关。
英文摘要
DESCRIPTION: (Adapted from the applicant's abstract.) While serious CMV
infections can involve a number of host tissues, the lungs are frequently
involved in lethal CMV infections. In these experiments several murine
models of CMV infection will be utilized in order to explore the cytokines,
TNF-a and lymphotoxin (TNF-b), as mediators of tissue damage. The role of
TNF-a and b in acute MCMV infection following sublethal and lethal virus
challenge will be examined. Using a model of acute CMV infection, the
amount of TNF expressed in tissue during lethal and sublethal virus
challenge will be compared and the effect of exogenous TNF on acute CMV
infection will be monitored. The effects of passive transfer of antibody
to TNF on virus replication will be determined. To explore the role of TNF
in lung injury, a murine model of pneumocystis associated MCMV and
graft-versus-host disease will be used, comparing the levels of TNF during
MCMV lung infection and pneumocystis. The production of TNF by lung cells
during pneumocystis will be examined and it will be determined if
production is antigen-specific or MHC-restricted. The effects of passive
transfer of antibody to TNF and lymphotoxin on MCMV pneumocystis will also
be determined. The Specific Aims of this project are: to determine the
role of TNF-a and lymphotoxin in acute MCMV infection; and to determine the
role of TNF-a and lymphotoxin in the pathogenesis of pneumocystis
associated with combined MCMV infection and GVH disease.
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The folate antagonist, methotrexate, is a potent inhibitor of murine and human cytomegalovirus in vitro.
叶酸拮抗剂甲氨蝶呤在体外是小鼠和人类巨细胞病毒的有效抑制剂。
DOI:
10.1016/0166-3542(89)90012-0
发表时间:
1989
期刊:
Antiviral research
影响因子:
7.6
作者:
[Shanley,JD, Debs,RJ]
通讯作者:
Debs,RJ
In vivo administration of monoclonal antibody to the NK 1.1 antigen of natural killer cells: effect on acute murine cytomegalovirus infection.
体内施用针对自然杀伤细胞 NK 1.1 抗原的单克隆抗体:对急性鼠巨细胞病毒感染的影响。
DOI:
10.1002/jmv.1890300113
发表时间:
1990
期刊:
Journal of medical virology
影响因子:
12.7
作者:
[Shanley,JD]
通讯作者:
Shanley,JD
Modification of acute murine cytomegalovirus adrenal gland infection by adoptive spleen cell transfer.
通过过继脾细胞移植改变急性小鼠巨细胞病毒肾上腺感染。
DOI:
10.1128/jvi.61.1.23-28.1987
发表时间:
1987
期刊:
Journal of virology
影响因子:
5.4
作者:
[Shanley,JD]
通讯作者:
Shanley,JD
Replication of human cytomegalovirus in cells deficient in beta 2-microglobulin gene expression.
人类巨细胞病毒在β2-微球蛋白基因表达缺陷的细胞中的复制。
DOI:
10.1099/0022-1317-75-10-2755
发表时间:
1994
期刊:
The Journal of general virology
影响因子:
--
作者:
[Wu,QH, Trymbulak,W, Tatake,RJ, Forman,SJ, Zeff,RA, Shanley,JD]
通讯作者:
Shanley,JD
Effects of L3T4+ lymphocyte depletion on acute murine cytomegalovirus infection.
L3T4 淋巴细胞耗竭对急性鼠巨细胞病毒感染的影响。
DOI:
10.1099/0022-1317-70-7-1765
发表时间:
1989
期刊:
The Journal of general virology
影响因子:
--
作者:
[Erlich,KS, Mills,J, Shanley,JD]
通讯作者:
Shanley,JD
共 7 条
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IMMUNE MODIFICATION OF CYTOMEGALOVIRUS INFECTION IN BONE MARROW
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CYTOMEGALOVIRUS PNEUMONITIS: THE ROLE OF TNF A AND B
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批准号:3348191
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CYTOMEGALOVIRUS PNEUMONITIS: THE ROLE OF TNF A AND B
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-
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