MAINTENANCE AND REPAIR OF VASCULAR ENDOTHELIUM

我们的具体目标是：1)进一步确定大脑和脑下垂体的特征 成纤维细胞生长因子已被FPLC纯化，通过测定 它们的主要结构。脑与垂体成纤维细胞生长因子的关系 也将使用针对这两种病毒的单克隆抗体来确定 分子。它们与内皮细胞生长因子的关系 (ECGF)将被确定。2)牛脑与牛脑的相互作用 垂体成纤维细胞生长因子与不同来源的血管内皮细胞 血管的区域和种类将被确定和成纤维细胞生长因子受体 特色化的。3)成纤维细胞生长因子对内皮细胞的影响 体外迁移及其作为趋化因子的可能作用 将会被确定。4)最后，使用抗成纤维细胞生长因子的单抗， 已知其在各种组织、肿瘤或培养细胞中的分布 体内含有血管生成因子会得到等式。下定决心。血管生成 成纤维细胞生长因子在三种不同体内模型(兔角膜、仓鼠)中的活性 尿囊和鸡胚绒毛尿囊膜)以及 成纤维细胞生长因子抗体在体内阻断这种活性的能力。其中的因素(S) 将测定模拟成纤维细胞生长因子作用的基底膜(BM)。 成纤维细胞生长因子对血管内皮生长因子合成和整合的影响 将分析培养的血管内皮细胞合成的BM。 骨髓基质和成纤维细胞生长因子对人牙髓细胞的维持和周转的影响 内皮细胞分裂所需的转铁蛋白受体，以及 在体内(红细胞生成系统)还是在器官培养(后肾 间充质)，已被证明是可诱导的，并与 将分析特定BM组件的外观。

Our specific aims are: 1) To further characterize brain and pituitary fibroblast growth factors (FGF) already purified by FPLC, by determining their primary structure. The relationship between brain and pituitary FGF will also be determined using monoclonal antibodies developed against both molecules. Their relationship with the endothelial cell growth factor (ECGF) will be determined. 2) The interaction of bovine brain and pituitary FGF with cultured endothelial cells originating from various vascular territories and species will be determined and the FGF receptors characterized. 3) The effect in vitro of FGF on endothelial cell migration, as well as its possible role as a chemotactic agent in vitro will be determined. 4) Finally, using monoclonal antibodies agaist FGF, its distribution in various tissues, neoplasms, or cultured cells known to contain angiogenic factors in vivo will be eq. determined. The angiogenic activity of FGF in three different in vivo models (rabbit cornea, Hamster pouch, and chick chorioallantoid membrane) will be analyzed as well as the ability of FGF antibodies to block such activity in vivo. The factor(s) in basement membranes (BM) which mimics the effect of FGF will be determined. The effect of FGF on the synthesis and integration of such factors in the BM synthesized by cultured vascular endothelial cells will be analyzed. The effect of both BM and FGF on the maintenance and turnover of the transferrin receptor which is required for endothelial cells to divide, and which in vivo (erythropoietic system), or in organ culture (metanephric mesenchyme), has been shown to be inducible and to correlate with the appearance of specific BM components will be analyzed.