REGENERATION OF CORNEAL ENDOTHELIUM

角膜内皮的再生

• 批准号: 3256545
• 负责人: DENIS J GOSPODAROWICZ
• 金额: $ 23.17万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3256545
• 关键词: affinity chromatography; angiogenesis; autoradiography; bioassay; cell migration; chick embryo; cornea disorder; corneal endothelium; corneal epithelium; cow; epidermal growth factor; extracellular matrix; eye injury; eye regeneration; fibroblast growth factor; high density lipoproteins; hybridomas; infant animal; iris; lens; membrane activity; monoclonal antibody; radioassay; radioimmunoassay; retinal pigment epithelium; tissue /cell culture; transfection; transferrin; wound healing

DESCRIPTION (applicant's abstract): The long term objectives are to determine the role of fibroblast growth factors (FGF) in the control of the proliferation and differentiation of the various ocular cell types which are sensitive to it and that of the newly discovered vascular endothelial cell growth factor (VEGF) in the control of proliferation and permeability of retina derived capillary endothelial (RCE) cells. The applicant would analyze whether transfection of iris melanocytes, RPE cells, and RCE cells, with bFRG cDNA expression vector could lead to their autonomous proliferation and ultimate transformation into neoplastic cells. Using both types of transfected cell types, he would first analyze whether bFGF acts intracellularly (as most oncogene products do) or needs to be released by the cells in order to interact with exposed cell surface receptors. Since cell transformation can be the direct result of oncogene expression, the applicant would analyze in transfected cells whether constitutive expression of c fos, and c myc oncogenes can be observed. He would initiate studies on the expression in ocular tissue of VEGF and study its possible participation in proliferative disorders occurring during diabetic retinopathy. To determine if expression of the VEGF gene or FGF gene had any effect during ontogeny, the applicant would inject AHR viruses containing those genes into avial embryos at different stages of development and analyze the occurrence of developmental abnormalities or tumors with the time of injection in ocular tissues. He would study the expression of VEGF in tissue and cells derived from the ocular system. If expressed, VEGF could play a role in controlling developmental processes. He would analyze the ability of VEGF to control events related to the angiogenic process in RCE cells. This includes its chemotactic activity and its ability to stimulate plasminogen activator and collagenase, while repressing plasminogen inhibitor activity. Finally the receptor for VEGF would be defined.

描述（申请人的摘要）：长期目标是 确定成纤维细胞生长因子（FGF）在控制中的作用 各种眼细胞类型的增殖和分化 对它和新发现的血管内皮细胞很敏感 细胞生长因子 (VEGF) 控制增殖和渗透性 视网膜来源的毛细血管内皮（RCE）细胞。 申请人将 分析是否转染虹膜黑素细胞、RPE细胞和RCE细胞， 与 bFRG cDNA 表达载体一起可以导致其自主 增殖并最终转化为肿瘤细胞。 使用 对于两种转染的细胞类型，他都会首先分析bFGF是否 在细胞内起作用（就像大多数癌基因产品一样）或需要释放 细胞为了与暴露的细胞表面受体相互作用。 由于细胞转化可能是癌基因表达的直接结果， 申请人将分析转染细胞中是否存在组成型 可以观察到c fos 和c myc 癌基因的表达。 他会 启动VEGF在眼组织表达的研究并研究其作用 可能参与糖尿病期间发生的增殖性疾病 视网膜病变。 确定 VEGF 基因或 FGF 基因的表达是否 个体发育期间如有任何影响，申请人将注射AHR病毒 将这些基因包含在不同阶段的禽胚胎中 发育并分析发育异常的发生或 肿瘤随眼组织注射时间的变化而变化。 他将研究 VEGF 在来自眼系统的组织和细胞中的表达。 如果 表达后，VEGF 可能在控制发育过程中发挥作用。 他将分析 VEGF 控制与 RCE细胞中的血管生成过程。 这包括其趋化活性 及其刺激纤溶酶原激活剂和胶原酶的能力，同时 抑制纤溶酶原抑制剂活性。 最后是VEGF的受体 将被定义。