REGENERATION OF CORNEAL ENDOTHELIUM

描述（申请人摘要）：长期目标是 确定成纤维细胞生长因子（FGF）在控制 各种眼细胞类型的增殖和分化， 对它和新发现的血管内皮细胞 细胞生长因子（VEGF）在增殖和渗透性控制中的作用 视网膜衍生的毛细血管内皮细胞（RCE）。 申请方 分析是否转染虹膜黑素细胞、RPE细胞和RCE细胞， 与bFRG cDNA表达载体的融合， 增殖并最终转化为肿瘤细胞。 使用 这两种类型的转染细胞类型，他将首先分析是否bFGF 在细胞内起作用（就像大多数癌基因产物一样）或需要释放 以与暴露的细胞表面受体相互作用。 由于细胞转化可能是癌基因表达的直接结果， 申请人将在转染的细胞中分析是否组成型 可观察到c-fos和c-myc癌基因表达。 他会 开展VEGF在眼组织中表达的研究， 可能参与糖尿病期间发生的增殖性疾病 视网膜病变 为了确定VEGF基因或FGF基因的表达是否 在个体发育期间的任何影响，申请人将注射AHR病毒 在不同的发育阶段， 发育和分析发育异常的发生，或 眼组织中的肿瘤随着注射时间的变化。 他将研究 VEGF在来源于眼系统的组织和细胞中的表达。 如果 VEGF的表达，VEGF可以在控制发育过程中发挥作用。 他将分析VEGF控制与肿瘤相关事件的能力， RCE细胞中的血管生成过程。 这包括它的趋化活性 以及其刺激纤溶酶原激活物和胶原酶的能力， 抑制纤溶酶原抑制剂活性。 最后，VEGF的受体 将被定义。

DESCRIPTION (applicant's abstract): The long term objectives are to determine the role of fibroblast growth factors (FGF) in the control of the proliferation and differentiation of the various ocular cell types which are sensitive to it and that of the newly discovered vascular endothelial cell growth factor (VEGF) in the control of proliferation and permeability of retina derived capillary endothelial (RCE) cells. The applicant would analyze whether transfection of iris melanocytes, RPE cells, and RCE cells, with bFRG cDNA expression vector could lead to their autonomous proliferation and ultimate transformation into neoplastic cells. Using both types of transfected cell types, he would first analyze whether bFGF acts intracellularly (as most oncogene products do) or needs to be released by the cells in order to interact with exposed cell surface receptors. Since cell transformation can be the direct result of oncogene expression, the applicant would analyze in transfected cells whether constitutive expression of c fos, and c myc oncogenes can be observed. He would initiate studies on the expression in ocular tissue of VEGF and study its possible participation in proliferative disorders occurring during diabetic retinopathy. To determine if expression of the VEGF gene or FGF gene had any effect during ontogeny, the applicant would inject AHR viruses containing those genes into avial embryos at different stages of development and analyze the occurrence of developmental abnormalities or tumors with the time of injection in ocular tissues. He would study the expression of VEGF in tissue and cells derived from the ocular system. If expressed, VEGF could play a role in controlling developmental processes. He would analyze the ability of VEGF to control events related to the angiogenic process in RCE cells. This includes its chemotactic activity and its ability to stimulate plasminogen activator and collagenase, while repressing plasminogen inhibitor activity. Finally the receptor for VEGF would be defined.