REGENERATION OF CORNEAL ENDOTHELIUM

通过放射免疫分析、放射受体分析、生物测定和 肝素-琼脂糖亲和层析，可能存在 各种眼组织(视网膜、角膜、视网膜色素沉着)中的成纤维细胞生长因子 将检查上皮、虹膜和晶状体)。可能的生产 由眼部各种肿瘤衍生的成纤维细胞生长因子将被检查。由于眼科 只有当特定的成纤维细胞生长因子受体部位是 目前，我们还将研究膜上是否存在成纤维细胞生长因子受体。 来自角膜上皮或视网膜的制剂。自从……出现以来 眼组织中的成纤维细胞生长因子和成纤维细胞生长因子受体都可能是 发育调节的过程，我们计划研究它们在 眼组织取自不同发育阶段的胚胎。 成纤维细胞生长因子与培养内皮细胞和视网膜来源的相互作用 将对毛细血管内皮细胞进行表征。成纤维细胞生长因子的纯化 将尝试通过亲和层析进行受体。成纤维细胞生长因子的作用 在体外对细胞迁移的影响(这一效应对视网膜尤为重要 毛细血管内皮细胞)及其对角膜的趋化活性 将测定内皮细胞或视网膜毛细血管内皮细胞。这个 成纤维细胞生长因子对癌基因激活的影响已被证明是 与细胞增殖(c-fos和c-myc)、细胞分化 (C Fos)和细胞寿命(C Ras)。因为眼角膜 内皮细胞外基质(ECM)已被证明具有同样的效力 作为支持细胞增殖的成纤维细胞生长因子，我们将从该底物中纯化 可能参与其促有丝分裂作用的因子(S)和 分析其与成纤维细胞生长因子的关系。因为高密度脂蛋白和转铁蛋白都有 已被证明是培养的角膜内皮细胞的进展因素 细胞，我们将描述它们在支持细胞生存方面的作用 它们支持细胞增殖的能力。

By means of a combination of RIA, radioreceptor assay, bioassay, and Heparin-Sepharose (HS) affinity chromatography, the possible presence of FGF in various ocular tissues (retina, cornea, retinal pigmented epithelium, iris, and lens) will be examined. The possible production of FGF by various tumors derived from the eye will be examined. Since ocular tissue would only be sensitive to FGF if specific FGF receptor sites are present, we will also study the presence of FGF receptors in membrane preparations from corneal epithelium or retina. Since the appearance of both FGF and of FGF receptors within ocular tissues could be a developmentally regulated process, we plan to study their appearance in ocular tissues obtained from embryo at different stages of development. the interaction of FGF with cultured endothelial cells and retina-derived capillary endothelial cells will be characterized. Purification of the FGF receptor by affinity chromatography will be attempted. The effect of FGF in vitro on cell migration (an effect particularly important for retinal capillary endothelial cells) and its chemotactic activity on corneal endothelial or retinal capillary endothelial cells will be determined. The effect of FGF on c oncogene activation, which has been shown to be correlated with cell proliferation (c fos and c myc), cell differentiation (c fos), and cell longevity (c ras), will be examined. Since the corneal endothelial cell extracellular matrix (ECM) has been shown to be as potent as FGF in supporting cell proliferation, we will purify from that substrate the factor(s) which could be involved in mediating its mitogenic effect and analyze its relationship with FGF. Since HDL and transferrin have both been shown to be progression factors for cultured corneal endothelial cells, we will delineate their roles in supporting cell survival versus their ability to support cell proliferation.