REGENERATION OF CORNEAL ENDOTHELIUM

角膜内皮的再生

• 批准号: 3256549
• 负责人: DENIS J GOSPODAROWICZ
• 金额: $ 19.95万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3256549
• 关键词: affinity chromatography; autoradiography; bioassay; cell migration; cornea disorder; corneal endothelium; corneal epithelium; epidermal growth factor; extracellular matrix; eye injury; eye regeneration; fibroblast growth factor; high density lipoproteins; hybridomas; infant animal; iris; laboratory mouse; lens; membrane activity; monoclonal antibody; radioassay; radioimmunoassay; retina; retinal pigment epithelium; tissue /cell culture; transferrin; wound healing

By means of a combination of RIA, radioreceptor assay, bioassay, and Heparin-Sepharose (HS) affinity chromatography, the possible presence of FGF in various ocular tissues (retina, cornea, retinal pigmented epithelium, iris, and lens) will be examined. The possible production of FGF by various tumors derived from the eye will be examined. Since ocular tissue would only be sensitive to FGF if specific FGF receptor sites are present, we will also study the presence of FGF receptors in membrane preparations from corneal epithelium or retina. Since the appearance of both FGF and of FGF receptors within ocular tissues could be a developmentally regulated process, we plan to study their appearance in ocular tissues obtained from embryo at different stages of development. the interaction of FGF with cultured endothelial cells and retina-derived capillary endothelial cells will be characterized. Purification of the FGF receptor by affinity chromatography will be attempted. The effect of FGF in vitro on cell migration (an effect particularly important for retinal capillary endothelial cells) and its chemotactic activity on corneal endothelial or retinal capillary endothelial cells will be determined. The effect of FGF on c oncogene activation, which has been shown to be correlated with cell proliferation (c fos and c myc), cell differentiation (c fos), and cell longevity (c ras), will be examined. Since the corneal endothelial cell extracellular matrix (ECM) has been shown to be as potent as FGF in supporting cell proliferation, we will purify from that substrate the factor(s) which could be involved in mediating its mitogenic effect and analyze its relationship with FGF. Since HDL and transferrin have both been shown to be progression factors for cultured corneal endothelial cells, we will delineate their roles in supporting cell survival versus their ability to support cell proliferation.

通过RIA、放射受体测定、生物测定和 肝素-琼脂糖（HS）亲和层析，可能存在的 各种眼组织（视网膜、角膜、视网膜色素沉着）中的FGF 上皮、虹膜和透镜）。 可能的生产 将检查源自眼睛的各种肿瘤的FGF。 由于眼部 只有当特定的FGF受体位点 目前，我们还将研究是否存在FGF受体在膜 角膜上皮或视网膜的制剂。 出现以来 眼组织内的FGF和FGF受体两者都可能是 发育调节过程，我们计划研究他们的外观， 从不同发育阶段的胚胎中获得的眼组织。 FGF与培养的内皮细胞和视网膜衍生的 将表征毛细血管内皮细胞。 FGF的纯化 将尝试通过亲和色谱法分离受体。 FGF的作用 在体外对细胞迁移（对视网膜神经元的作用 毛细血管内皮细胞）及其对角膜的趋化活性 将测定内皮或视网膜毛细血管内皮细胞。 的 FGF对c癌基因激活的作用，已被证明是 与细胞增殖（c fos和c myc）、细胞分化 （c fos）和细胞寿命（c ras）。 由于角膜 内皮细胞细胞外基质（ECM）已被证明是有效的， 作为支持细胞增殖的FGF，我们将从该底物中纯化 可能参与介导其促有丝分裂作用的因子， 分析其与FGF的关系。 由于高密度脂蛋白和转铁蛋白都具有 已被证明是培养的角膜内皮细胞的进展因素 细胞，我们将描绘他们的作用，支持细胞存活， 它们支持细胞增殖的能力。