Multiplexed bio-imaging mass spectrometry

多重生物成像质谱分析

• 批准号: DP170100036
• 负责人: Prof Philip Doble
• 金额: $ 29.08万
• 依托单位: University of Technology Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170100036
• 关键词: Multiplexed; bio; imaging; mass; spectrometry

This project aims to develop technology for simultaneous three-dimensional imaging of trace elements and molecules in biological tissues. This technology is an essential analytical and diagnostic advance to uncover latent mechanistic biology and will reveal subtle metabolic interactions between cells. The project will use laser ablation-inductively coupled plasma-mass spectrometry and lanthanide-conjugated probes to map the zinc metabolic pathway in the ageing mouse brain. It is expected to result in a complete imaging solution for quantitative spatial determination of all important elements, co-factors, metabolites and other functional molecules to investigate metabolic pathways which current technologies find difficult or impossible to track. Ultimately this technology is expected to underpin investigations of diseases where trace metal and chemical species dysfunction is implicated.

该项目旨在开发生物组织中微量元素和分子的同步三维成像技术。这项技术是一项重要的分析和诊断进步，可以揭示潜在的机械生物学，并将揭示细胞之间微妙的代谢相互作用。该项目将使用激光烧蚀电感耦合等离子体质谱和镧系元素共轭探针来绘制衰老小鼠大脑中的锌代谢途径。预计将产生一个完整的成像解决方案，用于所有重要元素、辅因子、代谢物和其他功能分子的定量空间测定，以研究目前技术难以或不可能跟踪的代谢途径。最终，这项技术有望支持对涉及痕量金属和化学物质功能障碍的疾病的调查。