Aptamer imaging mass spectrometry for biomarker quantification

用于生物标志物定量的适体成像质谱法

• 批准号: DP190102361
• 负责人: Prof Philip Doble
• 金额: $ 23.81万
• 依托单位: University of Technology Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190102361
• 关键词: Aptamer; imaging; mass; spectrometry; biomarker

This project aims to develop novel methods for quantifying bio-markers in histological specimens using aptamers, lanthanide visualising tags and laser ablation plasma mass spectrometry. Aptamers are short synthetic strands of nucleic acid with complex three dimensional structures that bind targets with exquisite specificity. This technology meets a major gap in current imaging modalities, has wide application to basic biology and diagnostics, and will eliminate the subjective interpretation of immunohistochemical stains.

该项目旨在开发新的方法，用于定量生物标记物在组织学标本中使用适体，镧系元素可视化标签和激光消融等离子体质谱。适体是具有复杂三维结构的短的合成核酸链，其以精确的特异性结合靶标。该技术满足了当前成像模式的主要差距，广泛应用于基础生物学和诊断学，并将消除免疫组织化学染色的主观解释。