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Bio-desalination: from cell to tap

生物海水淡化：从细胞到自来水

基本信息

批准号：
EP/J004871/1
负责人：
Anna Amtmann
金额：
$ 132.6万
依托单位：
University of Glasgow
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2011
资助国家：
英国
起止时间：
2011 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FJ004871%2F1
关键词：
Bio desalination cell tap

项目摘要

While three quarters of the earth's surface is covered in water almost all of it is present in the oceans with less than 0.5 % available as freshwater. Increasing global population, industrialisation and particularly agriculture exert significant pressures on this limited resource. With the aim to unlock the vast water resource in the oceans, attention for some time has focussed on the potential desalination of seawater to provide freshwater. However, current desalination technology, based on physicochemical processes, is a highly energy demanding process and its application is limited to fuel-rich and/or affluent developed countries. In this project we turn to biological mechanisms to remove sodium chloride (NaCl) from seawater ('bio-desalination'). We will exploit the fact that marine organisms employ energy-consuming transport processes to maintain low sodium concentrations inside their cells. The energy for this natural desalination ultimately comes from sunlight harvested by photo-autotrophic organisms at the bottom of the marine food chain. Based on available information on ion flux rates through individual transport proteins and their abundance in cell membranes, and taking into account the total cell surface area and volume generated by high-density bacterial cultures, we propose that the energized low-sodium internal volume of microbial cultures can be used as an ion exchanger to remove NaCl from the surrounding seawater.In a multi-pronged, integrated work programme led by a team of experts from different disciplines (microbiology, biophysics, molecular biology, environmental engineering and process engineering) we will generate the biological tools that will enable us to control membrane transport in marine bacteria, and we will design a simple and energy-efficient process for growth, exposure and removal of the bacterial cultures in/from the seawater. We will further maximise both the training potential and the potential impact of this innovative and multidisciplinary programme through staff exchange programmes, Social Impact Assessment and involvement of an Advisory Board which includes representatives of water industries and charities working in developing countries.The work comprises five work packages: 1.We will select a suitable isolate of marine cyanobacteria and identify environmental conditions (e.g. pH, carbon supply) that can act as on/off triggers for endogenous Na-export. 2. We will adjust the activity and biophysical properties of light-energized, retinal Cl-pumps and Na-channel proteins to generate a functional 'salt-accumulator for subsequent expression in the cyanobacteria under the control of an inducible promoter. 3. We will analyse the effect of environmental conditions (including salinity) on chemical and physical cell-wall properties and develop a controllable cell-aggregation protocol to facilitate rapid removal of the cyanobacteria from the desalted water. 4. We will assemble a prototype process engineering solution that combines the different biological phases of bio-desalination, and we will build a bench-scale model. 5. We will carry out a thorough assessment of social impact, demands, risks and policy implications of this new technology. The project addresses several fundamental challenges in different areas of modern biology and engineering. The groundbreaking advances made over recent years in synthetic biology and bioreactor technology have created an exciting research environment for tackling these challenges now with a realistic chance of success. Furthermore, bio-desalination technology lends itself to be combined with downstream industrial uses of the harvested microorganism e.g. the production of bio-fuel and extraction of bio-compounds for cosmetics and medicine. The potential benefit for society is evident as the proposed technology harvests the enormous energy that is encapsulated in autotrophic marine life, biological membranes and ion gradients.

虽然地球表面的四分之三被水覆盖，但几乎所有的水都存在于海洋中，只有不到0.5%的淡水可用。全球人口增长、工业化，特别是农业，对这一有限的资源造成了巨大压力。为了释放海洋中巨大的水资源，一段时间以来，人们的注意力集中在海水淡化以提供淡水的潜力上。然而，目前基于物理化学过程的海水淡化技术是一个高能耗过程，其应用仅限于燃料丰富和/或富裕的发达国家。在这个项目中，我们转向生物机制，从海水中去除氯化钠（NaCl）（“生物脱盐”）。我们将利用海洋生物利用耗能运输过程来维持细胞内低钠浓度的事实。这种自然淡化的能量最终来自海洋食物链底部的光自养生物所收获的阳光。基于通过单个转运蛋白的离子通量率及其在细胞膜中的丰度的可用信息，并考虑到高密度细菌培养物产生的总细胞表面积和体积，我们提出微生物培养物的通电低钠内部体积可以用作离子交换剂以从周围海水中去除NaCl。由不同学科专家小组领导的综合工作方案（微生物学、生物物理学、分子生物学、环境工程和过程工程）我们将开发生物工具，使我们能够控制海洋细菌的膜运输，我们将设计一个简单而节能的生长过程，在海水中/从海水中暴露和去除细菌培养物。我们将通过人员交流计划、社会影响评估和包括在发展中国家工作的水行业和慈善机构代表在内的咨询委员会的参与，进一步最大限度地发挥这一创新和多学科方案的培训潜力和潜在影响。1.我们将选择合适的海洋蓝藻分离物，并确定环境条件（例如pH值，碳供应），可以作为内源性钠输出的开关触发器。2.我们将调整光激发的视网膜Cl-泵和Na-通道蛋白的活性和生物物理性质，以产生一个功能性的盐累积器，用于在诱导型启动子的控制下在蓝藻中的后续表达。3.我们将分析环境条件（包括盐度）对化学和物理细胞壁特性的影响，并开发一种可控的细胞聚集协议，以促进快速去除的蓝藻从脱盐水。4.我们将组装一个原型工艺工程解决方案，结合生物脱盐的不同生物阶段，我们将建立一个实验室规模的模型。5.我们将对这项新技术的社会影响、需求、风险和政策影响进行全面评估。该项目解决了现代生物学和工程学不同领域的几个基本挑战。近年来在合成生物学和生物反应器技术方面取得的突破性进展为应对这些挑战创造了一个令人兴奋的研究环境，现在有了现实的成功机会。此外，生物脱盐技术本身可以与收获的微生物的下游工业用途相结合，例如生产生物燃料和提取用于化妆品和药物的生物化合物。对社会的潜在利益是显而易见的，因为拟议的技术收获了巨大的能量，这些能量被封装在自养海洋生物、生物膜和离子梯度中。