BIOLOGICAL EVALUATION OF A NEW DENTAL CEMENT

基于氧化锌和氧化锌混合物的新型牙科粘固剂配方 氧化铝粉末和含有邻乙氧基苯甲酸的液体组分 香草酸己酯已被开发用于牙科粘固剂。新的 水泥改善了机械和物理性能，并降低了用水量 溶解度，与更传统的氧化锌丁香酚或锌相比 氧化物-乙氧基苯甲酸粘固剂。而物理和机械的 对其性能和微观结构进行了表征，并对其生物学性能进行了研究 这种水泥的接受度还不得而知。这项研究的具体目的是 评价氧化锌-香草酸己酯的体内组织反应 水泥，采用两种试验方法。第一种方法是用油井 接受ADA建议的体内结缔组织植入试验 规格。粘固剂引发的组织反应将是 由定性的组织病理学检查作为以下功能进行监测 植入后时间。第二种评估方法涉及 盘状骨水泥标本植入兔腹膜腔内 老鼠。腹膜腔植入部位允许更彻底的 观察到的组织反应和组织反应之间的相互作用 水泥降解产物的形成。数量和数量 参与组织反应的细胞类型将作为 使用时间功能的腹膜腔灌洗技术。组织 植入腹膜腔后产生的胶囊将被 为组织学评估做好准备。腹膜腔技术将会 因此，将定量细胞效应与定性细胞效应相结合 牙科粘固剂组织反应的组织病理学研究。 原子吸收光谱分析和扫描电子显微镜 元素分析将被用来尝试确定降解物 可能导致异物的水泥制品 组织反应。这些生物学评估将作为筛查 描述对新型牙科粘固剂的组织反应的程序 强调有可能进行进一步研究的必要性。

A new dental cement formulation based on a mixture of zinc oxide and alumina powders and a liquid component containing ortho-ethoxybenzoic acid and hexyl vanillate has been developed for use as a dental cement. The new cement has improved mechanical and physical properties and low water solubility, as compared to more conventional zinc oxide eugenol or zinc oxide-ethxybenzoic acid cements. While the physical and mechanical properties and the microstructure have been characterized, the biological acceptance of the cement is unknown. The specific aim of this research is to evaluate the in vivo tissue reaction of the zinc oxide-hexyl vanillate cement, using two test methods. The first method consists of the well accepted in vivo connective tissue implantation test, as suggested by ADA specifications. The tissue reactions elicited by the cement will be monitored by qualitative histopathological examinations as a function of time after implantation. The second method of evaluation involves implantation of disc shaped cement specimens into the peritoneal cavity of rats. The peritoneal cavity implantation site allows for a more thorough investigation of the interaction between the observed tissue reactions and the formation of degradative products of the cement. The quantity and types of cells involved in the tissue reaction will be monitored as a function of time using the peritoneal cavity lavage technique. The tissue capsule resulting from implantation in the peritoneal cavity will be prepared for histological evaluation. The peritoneal cavity technique will therefore, combine the quantitative cellular effects and qualitative histopathological investigations of the tissue reaction to dental cements. Atomic absorption spectrophotometry and scanning electron microscopy and elemental analysis will be used in an attempt to identify degradative products of the cement which may have contributed to the foreign body tissue reaction. These biological evaluations will serve as screening procedures to describe the tissue reaction to the new dental cement and highlight the need for possible further research.