权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

BIOLOGICAL EVALUATION OF A NEW DENTAL CEMENT

新型牙科粘固剂的生物学评价

基本信息

批准号：
3424815
负责人：
JOHN C. KELLER
金额：
$ 1.19万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-03-01 至 1986-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3424815
关键词：
aluminum oxide atomic absorption spectrometry benzoates biomaterial evaluation dental adhesive /sealant histopathology peritoneum zinc

项目摘要

A new dental cement formulation based on a mixture of zinc oxide and alumina powders and a liquid component containing ortho-ethoxybenzoic acid and hexyl vanillate has been developed for use as a dental cement. The new cement has improved mechanical and physical properties and low water solubility, as compared to more conventional zinc oxide eugenol or zinc oxide-ethxybenzoic acid cements. While the physical and mechanical properties and the microstructure have been characterized, the biological acceptance of the cement is unknown. The specific aim of this research is to evaluate the in vivo tissue reaction of the zinc oxide-hexyl vanillate cement, using two test methods. The first method consists of the well accepted in vivo connective tissue implantation test, as suggested by ADA specifications. The tissue reactions elicited by the cement will be monitored by qualitative histopathological examinations as a function of time after implantation. The second method of evaluation involves implantation of disc shaped cement specimens into the peritoneal cavity of rats. The peritoneal cavity implantation site allows for a more thorough investigation of the interaction between the observed tissue reactions and the formation of degradative products of the cement. The quantity and types of cells involved in the tissue reaction will be monitored as a function of time using the peritoneal cavity lavage technique. The tissue capsule resulting from implantation in the peritoneal cavity will be prepared for histological evaluation. The peritoneal cavity technique will therefore, combine the quantitative cellular effects and qualitative histopathological investigations of the tissue reaction to dental cements. Atomic absorption spectrophotometry and scanning electron microscopy and elemental analysis will be used in an attempt to identify degradative products of the cement which may have contributed to the foreign body tissue reaction. These biological evaluations will serve as screening procedures to describe the tissue reaction to the new dental cement and highlight the need for possible further research.

一种新的牙科粘固剂配方，氧化铝粉末和含有邻乙氧基苯甲酸的液体组分香草酸己酯已被开发用作牙科粘固剂。新水泥具有改善的机械和物理性能，溶解度，与更常规的氧化锌丁香酚或锌相比氧化物-乙基苯甲酸粘固剂。虽然物理和机械性能和微观结构进行了表征，生物水泥的接受程度尚不清楚。本研究的具体目的是评价氧化锌-香草酸己酯的体内组织反应使用两种测试方法。第一种方法包括根据ADA的建议，接受体内结缔组织植入试验规范. 骨水泥引起的组织反应将通过定性组织病理学检查进行监测，植入后时间。第二种评估方法涉及将盘形骨水泥样本植入腹膜腔，大鼠腹膜腔植入部位允许更彻底的研究观察到的组织反应与水泥降解产物的形成。数量和参与组织反应的细胞类型将被监测，腹腔灌洗技术的时间函数。组织将植入腹膜腔中产生的囊制备用于组织学评价。腹腔技术将因此，联合收割机将定量细胞效应和定性组织对牙科粘固剂反应的组织病理学研究。原子吸收分光光度法和扫描电镜，将使用元素分析来尝试识别降解可能导致异物的骨水泥产品组织反应这些生物学评价将作为筛选描述组织对新型牙科粘固剂反应的程序，强调有必要进行进一步的研究。