GABA SYNAPTIC CIRCUITS IN CARDIOVASCULAR NUCLEI

心血管核中的 GABA 突触回路

• 批准号: 3407828
• 负责人: BRUCE E MALEY
• 金额: $ 5.6万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3407828
• 关键词: autonomic nervous system; autoradiography; cardiovascular function; electron microscopy; gamma aminobutyrate; histochemistry /cytochemistry; immunochemistry; medulla oblongata; microscopy; neural information processing; neuroanatomy; neurophysiology; neurotransmitters; respiratory function; solitary tract nucleus; synapses; tritium

Using a new antibody directed solely against the GABA molecule itself and receptor binding autoradiography, the long term objective of this proposed study is to determine the organization and function of GABAergic circuitry in specific neural regions of the autonomic nervous system, which are involved in regulation and/or modulation of cardiovascular and respiratory functions. The specific aims of this proposal include: 1) to investigate thoroughly at the light microscopic level in the cat immunohistochemical distribution of GABA containing neurons, fibers and terminals in the following autonomic nervous system nuclei: the nucleus tractus solitarii and the rostral ventral medullary area (of Schlaefke); 2) to study the transmitter coded circuitry of the GABAergic system at the ultrastructural level in the nucleus tractus solitarii and the rostral ventral medullary area (of Schlaefke) in order to determine the specific patterns of synaptic circuits concerning the localization of GABA receptors using (3H) muscimol in tractus solitarii and the rostral ventral medullary area of Schlaefke. The results of these studies will provide the first immunohistochemical and receptor binding evidence of the specific GABAergic circuits involved in autonomic nervous system nuclei. In addition, these studies will furnish an understanding of the neurobiology of GABAergic neurons which will contribute important information concerning the cells and circuits that are centrally involved in the regulation of cardiovascular and respiratory events such as blood pressure, heart rate and respiratory depression.

使用一种专门针对GABA分子本身的新抗体 受体结合放射自显影，建议的长期目标 研究是确定GABA能回路的组织和功能 在自主神经系统的特定神经区域，这些区域是 参与调节和/或调节心血管和呼吸系统 功能。这项建议的具体目的包括：1)调查 在光镜下彻底观察猫的免疫组织化学 含GABA的神经元、纤维和终末在下丘脑的分布 自主神经系统核团：孤束核 和(Schlaefke)头端延髓腹侧区；2)研究 伽玛能系统超微结构的发射机编码电路 孤束核和延髓吻侧腹侧的水平 (Schlaefke)区，以确定突触的具体模式 用~(~H)蝇香酚定位GABA受体的电路 孤束和Schlaefke头端延髓腹侧区。 这些研究的结果将提供第一个免疫组织化学和 受体结合证据的特定GABA能回路参与 自主神经系统核团。此外，这些研究将提供 了解GABA能神经元的神经生物学 提供有关细胞和电路的重要信息 集中参与心血管和呼吸系统的调节 血压、心率和呼吸抑制等事件。