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GABA SYNAPTIC CIRCUITS IN CARDIOVASCULAR NUCLEI

心血管核中的 GABA 突触回路

基本信息

批准号：
3407829
负责人：
BRUCE E MALEY
金额：
$ 5.39万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-07-01 至 1989-06-30
项目状态：
已结题

项目摘要

Using a new antibody directed solely against the GABA molecule itself and receptor binding autoradiography, the long term objective of this proposed study is to determine the organization and function of GABAergic circuitry in specific neural regions of the autonomic nervous system, which are involved in regulation and/or modulation of cardiovascular and respiratory functions. The specific aims of this proposal include: 1) to investigate thoroughly at the light microscopic level in the cat immunohistochemical distribution of GABA containing neurons, fibers and terminals in the following autonomic nervous system nuclei: the nucleus tractus solitarii and the rostral ventral medullary area (of Schlaefke); 2) to study the transmitter coded circuitry of the GABAergic system at the ultrastructural level in the nucleus tractus solitarii and the rostral ventral medullary area (of Schlaefke) in order to determine the specific patterns of synaptic circuits concerning the localization of GABA receptors using (3H) muscimol in tractus solitarii and the rostral ventral medullary area of Schlaefke. The results of these studies will provide the first immunohistochemical and receptor binding evidence of the specific GABAergic circuits involved in autonomic nervous system nuclei. In addition, these studies will furnish an understanding of the neurobiology of GABAergic neurons which will contribute important information concerning the cells and circuits that are centrally involved in the regulation of cardiovascular and respiratory events such as blood pressure, heart rate and respiratory depression.

使用一种新的抗体，只针对GABA分子本身，受体结合放射自显影，这一建议的长期目标研究的目的是确定GABA能回路的组织和功能在自主神经系统的特定神经区域，参与心血管和呼吸系统的调节和/或调节功能协调发展的这项建议的具体目的包括：1）调查在猫免疫组织化学的光镜水平上， GABA能神经元、纤维和终末的分布以下自主神经系统核：孤束核和（Schlaefke的）头端腹侧延髓区; 2）研究超微结构中GABA能系统的发射器编码电路孤束核和延髓头端腹侧水平区域（Schlaefke），以确定突触的特定模式使用（3 H）蝇蕈醇定位GABA受体的相关回路孤束和Schlaefke延髓头端腹侧区。这些研究的结果将提供第一个免疫组织化学和受体结合证据的具体GABA能电路参与自主神经系统核团此外，这些研究将提供对GABA能神经元的神经生物学的理解，提供了关于细胞和电路的重要信息，主要参与心血管和呼吸系统的调节血压、心率和呼吸抑制等事件。