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ENKEPHALIN & SEROTONIN CIRCUITS IN CARDIOVASCULAR NUCLEI

脑啡肽

基本信息

批准号：
3448526
负责人：
BRUCE E MALEY
金额：
$ 4.45万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
1983
资助国家：
美国
起止时间：
1983-07-01 至 1986-06-30
项目状态：
已结题

项目摘要

The long term objective of this proposed study is to determine the organization and anatomical relationships of enkephalin and serotonin circuits in a specific cardiovascular nucleus, the nucleus tractus solitarii, as they possibly relate to regulation of cardiovascular functions. The specific aims of this proposal are 1) to determine the precise termination of enkephalin immunoreactive neurons which are contained within cardiovascular related subdivisions of the nucleus tractus solitarii of the cat, 2) to investigate the synaptic relationships and morphological aspects of enkephalin immunoreactive terminals and neurons as they relate to visceral afferents terminating in the nucleus tractus solitrarii and 3) to describe the synaptic relationships and patterns of serotonergic terminals and enkephalin containing neurons and terminals. In the case of possible projections of enkephalin containing neurons and serotonergic input to these neurons, the concurrent use of HRP retrograde tract-tracing will be used in combination with neurotransmitter immunohistochemistry both at the light and electron microscopic levels. Results of the proposed study are expected to provide the morphological basis for the enkephalinergic and serotonergic effects observed within the nucleus tractus solitarii and its projections in relation to central regulation of cardiovascular function. Furthermore, these studies will contribute important information concerning the neurons and circuits that are involved in central control of autonomic functions.

这项拟议研究的长期目标是确定脑啡肽和血清素的组织和解剖关系特定心血管核（束核）中的回路 solitarii，因为它们可能与心血管调节有关功能。该提案的具体目标是 1) 确定精确终止脑啡肽免疫反应神经元包含在与心血管相关的束核细分内猫的 solitarii，2) 研究突触关系和脑啡肽免疫反应末端和神经元的形态学方面它们与终止于束核的内脏传入有关 solitrarii 和 3) 描述突触关系和模式血清素能末梢和含有脑啡肽的神经元和末梢。在含有脑啡肽的神经元可能投射的情况和这些神经元的血清素输入，同时使用 HRP 逆行纤维束追踪将与神经递质结合使用光镜和电子显微镜水平的免疫组织化学。拟议研究的结果预计将提供形态学观察到的脑啡肽能和血清素能效应的基础孤束核及其相对于中枢的投影心血管功能的调节。此外，这些研究将提供有关神经元和电路的重要信息参与自主功能的中央控制。