Laser-printable point-of-care sensors for low-cost medical diagnosis and disease monitoring

用于低成本医疗诊断和疾病监测的激光打印床旁传感器

• 批准号: EP/K023454/1
• 负责人: Robert Eason
• 金额: $ 23.71万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FK023454%2F1
• 关键词: Laser; printable; point; care; sensors

The research we are proposing here is to develop laser-printed paper-based sensors that will satisfy the criteria of being robust, inexpensive, user-friendly, disposable, and easy to deliver to those patients and members of the public who need to access point-of-care diagnosis. Such sensors would find a ready market within the healthcare and personalised medicine areas, and would prove invaluable when rapid deployment and testing is required in the case of epidemics or worse still, pandemics. The proposal here is a first feasibility trial to confirm that we can indeed print viable biological material, for application in detection of selected breast-cancer biomarkers. The markers have been identified and validated by our IfLS/FOM team and are used to study the response of patients receiving chemotherapy. This would represent an original diagnostic product developed at the UoS. The work not only involves laser-based printing, and evaluating post-printing the viability of the printed biomaterial, but also investigating techniques for machining and processing of the paper substrate to be used for the sensor. There are many advantage of this laser-printing approach that are uniquely appropriate for non-contact delivery of biomedical materials, namely:Flexible processing (patterning and deposition) by simple changes to laser parameters.Non-contact processing (patterning and printing) which is vital when dealing with biological agents.No need for a specialist environment thereby eliminating unwarranted processing costs.The inherent speed of laser printing: a direct analogy can be drawn from current laser printers which can print many sheets per second.low production costs because of the possibility to mass-produce devices on a roll-to-roll scale.High feature resolution allowing the creation of compact devices. (Our final goal in a future follow-up proposal will be to print biomaterials in the form of bar-codes for intelligent/active diagnostics readout via smart phone applications).This laser-printing requires much smaller reagent/analyte volumes compared to current liquid based delivery (printing) methods.

我们在这里提出的研究是开发激光打印的纸基传感器，这些传感器将满足以下标准：坚固耐用、价格低廉、用户友好、一次性使用，并且易于交付给需要进行即时诊断的患者和公众。这种传感器将在医疗保健和个性化医疗领域找到一个现成的市场，并且在流行病或更糟糕的流行病情况下需要快速部署和测试时将证明是非常宝贵的。该提案是第一个可行性试验，以确认我们确实可以打印可行的生物材料，用于检测选定的乳腺癌生物标志物。这些标志物已经被我们的IfLS/FOM团队鉴定和验证，并用于研究接受化疗的患者的反应。这将代表UoS开发的原始诊断产品。这项工作不仅涉及基于激光的打印，以及打印后评估打印生物材料的可行性，而且还研究了用于传感器的纸基材的加工和处理技术。这种激光打印方法有许多独特的优点，特别适用于生物医学材料的非接触式输送，即：通过简单地改变激光参数，可以实现（图案化和沉积）工艺。（图案化和打印），这在处理生物制剂时至关重要。无需专业环境，从而消除了不必要的加工成本。激光打印的固有速度：可以从目前每秒可以打印许多张的激光打印机中得出直接的类比。由于可以在卷到卷上批量生产设备，高特征分辨率允许创建紧凑的设备。(Our未来后续提案的最终目标是以条形码的形式打印生物材料，用于通过智能电话应用程序进行智能/主动诊断读出）。与当前基于液体的递送（打印）方法相比，这种激光打印需要小得多的试剂/分析物体积。

项目成果

Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer.

• DOI: 10.1063/1.4878696
• 发表时间: 2014-05
• 期刊: Biomicrofluidics
• 影响因子: 3.2
• 作者: I. Katis;J. Holloway;J. Madsen;S. Faust;S. Garbis;Peter J. Smith;D. Voegeli;D. Bader;R. Eason;C. Sones

Capillary-based reverse transcriptase loop-mediated isothermal amplification for cost-effective and rapid point-of-care COVID-19 testing.

• DOI: 10.1016/j.aca.2021.339002
• 发表时间: 2021-11-15
• 期刊: Analytica chimica acta
• 影响因子: 6.2
• 作者: John AJUK;He PJW;Katis IN;Galanis PP;Iles AH;Eason RW;Sones CL
• 通讯作者: Sones CL

Laser direct writing of programmable delays for fabrication of paper-based sensors that allow CRP detection

激光直写可编程延迟，用于制造允许 CRP 检测的纸基传感器

• 发表时间: 2016
• 作者: He P.J.W,

Direct-write laser techniques for the manufacture of multiplexed paper-based diagnostic sensors

用于制造多路纸基诊断传感器的直写激光技术

• 作者: Katis, I.N.

Rapid prototyping of microfluidic channels in nitrocellulose using laser-direct-write patterning

使用激光直写图案快速制作硝化纤维中微流体通道的原型

• 作者: He, Peijun