OPTICAL STUDIES OF NEURON FUNCTION

神经元功能的光学研究

• 批准号: 3410713
• 负责人: KATHERINE GRAUBARD
• 金额: $ 10.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3410713
• 关键词: Malacostraca; action potentials; axon; calcium transporting ATPase; dendrites; electrophysiology; electrostimulus; ganglions; intercellular connection; membrane channels; neural transmission; neurons; neuropil; neurotransmitters; saltwater environment; synapses

The long-term objective of this study is to derive general principles for the functioning of local circuit neurons, especially those cells which have synaptic output from their integrating regions (e.g., dendro-dendritic synapses) and which may use graded synaptic transmission below the threshold for spikes. The immediate objective is to understand the functioning of an invertebrate system, the stomatogastric ganglion of the crab Cancer borealis. The focus of this study will be experiments designed to elucidate the role of calcium in stomatogastric cell function. Questions will include: 1) When is voltage-dependent calcium influx due mostly to graded voltage changes (the pyloric rhythm) and when is it due mostly to spikes? Does calcium influx vary with the cell type, the location within the neuron, or with synaptic or neuromodulator activity? 2) How do neuromodulators affect the resting internal calcium concentration and the voltage- dependent calcium influx in different regions of identified cells? Methods to be used in this study include: intracellular somatic and neuropil recording, simultaneous measurements of internal calcium fluctuations in different regions of single neurons by use of the calcium indicator dye Arsenazo III and a photodiode array, and measurements of resting internal calcium concentrations with Fura 2 and CCD camera. Of necessity, most studies of neuron function have been constrained spatially: intracellular electrodes are placed in only one or two simultaneous locations in single cells, typically the soma. This study will provide an opportunity to look at the behavior of an entire neuron during its normal range of behaviors as it uses what might be considered its most interesting ion, calcium.

这项研究的长期目的是推导一般 局部电路神经元功能的原理，尤其是 那些从其积分中具有突触输出的单元 区域（例如Dendro Dendritic Synapses），可以使用 尖峰阈值以下的分级突触变速箱。 这 直接目标是了解一个 无脊椎动物系统，螃蟹的气孔神经节 癌症。 这项研究的重点将是实验 旨在阐明钙在大胃细胞中的作用 功能。 问题将包括：1）何时依赖电压 钙涌入主要是由于电压变化而造成的（幽门 节奏）以及什么时候主要是由于峰值？ 钙涌入 随细胞类型，神经元内的位置或 突触或神经调节剂活性？ 2）神经调节剂如何 影响静止的内部钙浓度和电压 - 鉴定细胞的不同区域的依赖性钙涌入？ 本研究中使用的方法包括：细胞内躯体和 神经记录，同时测量内部 单神经元不同区域的钙波动通过使用 钙指示剂染料Arsenazo III和光电二极管阵列的 以及与静息内部钙浓度的测量 Fura 2和CCD相机。 必要时，大多数神经元的研究 功能已在空间上受到约束：细胞内电极 仅放置在一个或两个同时位于单个位置 细胞，通常是躯体。 这项研究将提供机会 查看正常范围内整个神经元的行为 行为，因为它使用了可能被认为是最多的行为 有趣的离子，钙。

项目成果

Spatially and temporally resolved calcium concentration changes in oscillating neurons of crab stomatogastric ganglion.

蟹口胃神经节振荡神经元的空间和时间分辨钙浓度变化。

• DOI: 10.1073/pnas.86.5.1679
• 发表时间: 1989
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Ross,WN;Graubard,K
• 通讯作者: Graubard,K

Nickel induces oscillatory behavior and enhanced synaptic and electrotonic transmission between stomatogastric neurons of Panulirus interruptus.

镍诱导振荡行为并增强中断龙虾口胃神经元之间的突触和电紧张传递。

• DOI: 10.1016/0006-8993(93)91087-9
• 发表时间: 1993
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Zirpel,L;Baldwin,D;Graubard,K
• 通讯作者: Graubard,K