GRADED SYNAPTIC TRANSMISSION IN LOCAL NEURAL CIRCUITS

局部神经回路中的分级突触传递

• 批准号: 3396417
• 负责人: KATHERINE GRAUBARD
• 金额: $ 10.59万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396417
• 关键词: dendrites; electrophysiology; evoked potentials; ganglions; neural information processing; neural plasticity; neural transmission; neuroanatomy; saltwater environment; synapses

The objective of the proposed research is to increase our understanding of the synaptic mechanisms used by local circuit neurons and, in particular, to study the function of graded synaptic transmission (GST) in spiking, local circuit neurons. The immediate objective is to understand the functioning of GST in an invertebrate model system, the stomatogastric ganglion of the spiny lobster, Panulirus interruptus. This study of local circuit neurons will describe neuron-to-neuron GST behavior and compare it to the behavior of spike-evoked postsynaptic potentials (PSPs) in the same neurons. A detailed study of the mechanisms underlying GST and a comparison to those of PSPs will focus on voltage dependent ionic currents (e.g., presynaptic calcium current). Attention will be given to the plastic properties of synapses, including time dependent effects. Methods to be used in this study include intracellular somatic and neuropil recording and whole cell voltage clamp techniques. Little is known about GST and less about GST as it occurs in spiking neurons. GST and PSP properties and their underlying mechanisms have never previously been examined in neurons which use both GST and PSPs as a part of normal function. However, many cells involved in local neuronal circuits in the major invertebrate phyla and in mammals (including man) probably use both GST and PSPs as methods of synaptic communication. This research should be a first step toward understanding how these neurons function.

拟议研究的目的是增加我们对 局部电路神经元使用的突触机制，尤其是 为了研究分级突触传播（GST）在峰值中的功能， 局部电路神经元。 直接目标是了解 GST在无脊椎动物模型系统中的功能，气孔胃 刺龙虾的神经节，Panulirus Intruptus。 这项本地研究 电路神经元将描述神经元到神经元的GST行为并进行比较 在同一的尖峰诱发的突触后电位（PSP）的行为 神经元。 对商品及服务税和A的机制的详细研究 与PSP的比较将集中在依赖电压的离子电流上 （例如，突触前钙电流）。 注意 突触的塑性特性，包括时间依赖性效应。 方法 在本研究中使用的包括细胞内体细胞和神经胶质 记录和全细胞电压夹技术。 对 GST越来越涉及GST，因为它发生在尖峰神经元中。 GST和PSP 属性及其基本机制以前从未有过 在使用GST和PSP作为正常的一部分的神经元中检查 功能。 但是，许多参与局部神经元回路的细胞 主要的无脊椎动物门和哺乳动物（包括人）可能同时使用 GST和PSP作为突触通信的方法。 这项研究应该是 了解这些神经元如何发挥作用的第一步。