GRADED SYNAPTIC TRANSMISSION IN LOCAL NEURAL CIRCUITS

局部神经回路中的分级突触传递

• 批准号: 3396421
• 负责人: KATHERINE GRAUBARD
• 金额: $ 10.19万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396421
• 关键词: Malacostraca; dendrites; electrophysiology; evoked potentials; ganglions; neural information processing; neural plasticity; neural transmission; neuroanatomy; saltwater environment; synapses

The objective of the proposed research is to increase our understanding of the synaptic mechanisms used by local circuit neurons and, in particular, to study the function of graded synaptic transmission (GST) in spiking, local circuit neurons. The immediate objective is to understand the functioning of GST in an invertebrate model system, the stomatogastric ganglion of the spiny lobster, Panulirus interruptus. This study of local circuit neurons will describe neuron-to-neuron GST behavior and compare it to the behavior of spike-evoked postsynaptic potentials (PSPs) in the same neurons. A detailed study of the mechanisms underlying GST and a comparison to those of PSPs will focus on voltage dependent ionic currents (e.g., presynaptic calcium current). Attention will be given to the plastic properties of synapses, including time dependent effects. Methods to be used in this study include intracellular somatic and neuropil recording and whole cell voltage clamp techniques. Little is known about GST and less about GST as it occurs in spiking neurons. GST and PSP properties and their underlying mechanisms have never previously been examined in neurons which use both GST and PSPs as a part of normal function. However, many cells involved in local neuronal circuits in the major invertebrate phyla and in mammals (including man) probably use both GST and PSPs as methods of synaptic communication. This research should be a first step toward understanding how these neurons function.

拟议研究的目的是增加我们对 局部回路神经元使用的突触机制，特别是， 为了研究分级突触传递（GST）在发放中的作用， 局部回路神经元 当前的目标是了解 GST在无脊椎动物模型系统中的功能， 龙虾的神经节，Panulirus interruptus。 这项研究，当地 电路神经元将描述神经元到神经元的GST行为，并将其 的行为，棘波诱发的突触后电位（PSP）在同一 神经元 对GST的机制进行了详细研究， 与PSP的比较将集中在电压依赖性离子电流上 (e.g.,突触前钙电流）。 将注意到 突触的可塑性，包括时间依赖效应。 方法 包括细胞内体细胞和神经细胞 记录和全电池电压箝位技术。 知之甚少 GST和较少关于GST，因为它发生在尖峰神经元。 GST和PSP 这些特性及其潜在机制以前从未被 在使用GST和PSP作为正常神经元的一部分的神经元中进行了检查。 功能 然而，许多参与局部神经元回路的细胞在大脑中， 主要的无脊椎动物门和哺乳动物（包括人类）可能都使用 GST和PSP作为突触通信的方法。 这项研究应该是 这是了解这些神经元功能的第一步。