PHYCOBILIN GENE STRUCTURE AND REGULATION

藻胆蛋白基因结构和调控

• 批准号: 3438483
• 负责人: Rona L. Hirschberg
• 金额: $ 7.2万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3438483
• 关键词: Cyanophyta; bacterial genetics; fluorescence spectrometry; gene expression; genetic mapping; messenger RNA; microorganism metabolism; nitrogen fixation; nucleic acid structure; photosynthetic bacteria

Phycobilins are the major accessory photosynthetic pigments of cyanobacteria. They are found in structures call phycobilisomes which lie on the surface of the thylakoid membranes and may comprise over 50% of the cell protein. Phycobilins also serve as a large reserve of amino nitrogen in these organisms, and the proteins are degraded during nitrogen starvation. This is particularly important in nitrogen-fixing cyanobacteria, since it supports synthesis of nitrogenase and the other proteins needed for nitrogen fixation and heterocyst development. Our long term goals for research on phycobilins are to identify and characterize the genes for phycobilins and related proteins which constitute phycobilisomes and to understand how the genes are regulated in relation to nitrogen metabolism in nitrogen-fixing cyanobacteria. This work is one part of more broadly based studies we are conducting on the molecular biology of nitrogen metabolism in cyanobacteria. The specific aims of the studies proposed here are to identify and characterize the genes for the major phycobilins of Anabaena variabilis 29413 and to study their regulation in relation to different states of nitrogen metabolism, especially nitrogen starvation. We will isolate genes from our Ch30- Anabaena library with heterologous probes and/or with synthetic oligonucleotides. The genes will be sequenced, and their organization in the genome will be determined. We will use hybridization techniques, pulse labeling, and spectroscopic analysis to study regulation of phycobilin gene labeling, and spectroscopic analysis to study regulation of phycobilin gene expression. Several different nitrogen sources, as well as nitrogen starvation, will be examined for their effect on mRNA and protein levels.

藻胆素是植物的主要辅助光合色素

项目成果

Structure and regulation of genes encoding phycocyanin and allophycocyanin from Anabaena variabilis ATCC 29413.

多变鱼腥藻 ATCC 29413 藻蓝蛋白和别藻蓝蛋白编码基因的结构和调控。

• DOI: 10.1128/jb.170.4.1858-1865.1988
• 发表时间: 1988
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Johnson,TR;Haynes2nd,JI;Wealand,JL;Yarbrough,LR;Hirschberg,R
• 通讯作者: Hirschberg,R