PHYCOBILIN GENE STRUCTURE AND REGULATION
PHYCOBILIN GENE STRUCTURE AND REGULATION
批准号:
3438483
负责人:
Rona L. Hirschberg
金额:
$7.2万
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-07-01 至 1989-12-31
中文摘要
藻胆素是植物的主要辅助光合色素
英文摘要
Phycobilins are the major accessory photosynthetic pigments of
cyanobacteria. They are found in structures call phycobilisomes
which lie on the surface of the thylakoid membranes and may
comprise over 50% of the cell protein. Phycobilins also serve as a
large reserve of amino nitrogen in these organisms, and the
proteins are degraded during nitrogen starvation. This is
particularly important in nitrogen-fixing cyanobacteria, since it
supports synthesis of nitrogenase and the other proteins needed
for nitrogen fixation and heterocyst development. Our long term
goals for research on phycobilins are to identify and characterize
the genes for phycobilins and related proteins which constitute
phycobilisomes and to understand how the genes are regulated in
relation to nitrogen metabolism in nitrogen-fixing cyanobacteria.
This work is one part of more broadly based studies we are
conducting on the molecular biology of nitrogen metabolism in
cyanobacteria. The specific aims of the studies proposed here are
to identify and characterize the genes for the major phycobilins
of Anabaena variabilis 29413 and to study their regulation in
relation to different states of nitrogen metabolism, especially
nitrogen starvation. We will isolate genes from our Ch30-
Anabaena library with heterologous probes and/or with synthetic
oligonucleotides. The genes will be sequenced, and their
organization in the genome will be determined. We will use
hybridization techniques, pulse labeling, and spectroscopic
analysis to study regulation of phycobilin gene labeling, and
spectroscopic analysis to study regulation of phycobilin gene
expression. Several different nitrogen sources, as well as nitrogen
starvation, will be examined for their effect on mRNA and
protein levels.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Structure and regulation of genes encoding phycocyanin and allophycocyanin from Anabaena variabilis ATCC 29413.
多变鱼腥藻 ATCC 29413 藻蓝蛋白和别藻蓝蛋白编码基因的结构和调控。
DOI:
10.1128/jb.170.4.1858-1865.1988
发表时间:
1988
期刊:
Journal of bacteriology
影响因子:
3.2
作者:
[Johnson,TR, Haynes2nd,JI, Wealand,JL, Yarbrough,LR, Hirschberg,R]
通讯作者:
Hirschberg,R
MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM
-
批准号:2282840
-
项目类别:
-
资助金额:$2.0万
-
财政年份:1995
-
负责人:Rona L. Hirschberg
-
依托单位:
PILIN GENES OF EIKENELLA CORRODENS
-
批准号:2131343
-
项目类别:
-
资助金额:$2.18万
-
财政年份:1992
-
负责人:Rona L. Hirschberg
-
依托单位:
PILIN GENES OF EIKENELLA CORRODENS
-
批准号:3425840
-
项目类别:
-
资助金额:$4.92万
-
财政年份:1992
-
负责人:Rona L. Hirschberg
-
依托单位:
MOLECULAR GENETIC STUDIES OF EIKENELLA CORRODENS
-
批准号:3436836
-
项目类别:
-
资助金额:$10.8万
-
财政年份:1990
-
负责人:Rona L. Hirschberg
-
依托单位:
SMALL INSTRUMENTATION GRANT
-
批准号:3524124
-
项目类别:
-
资助金额:$1.08万
-
财政年份:1990
-
负责人:Rona L. Hirschberg
-
依托单位:
海外基金