BRAIN TISSUE TRANSPLANTS AND REPRODUCTIVE PROCESSES

脑组织移植和生殖过程

• 批准号: 3447955
• 负责人: GARY W ARENDASH
• 金额: $ 4.92万
• 依托单位: UNIVERSITY OF SOUTH FLORIDA
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3447955
• 关键词: autoradiography; brain cell; cell differentiation; immature animal; luteinizing hormone; nervous system transplantation; psychophysiology; reproductive system; sex behavior; silver impregnation; testosterone

Although brain tissue transplantation in rats has recently been used to investigate the development and potential for functional integration of transplanted brain regions, exploring reproductively-important brain processes with this new research tool has not yet been cohesively addressed. In this regard, little is known about the exact mechanism(s) by which gonadal hormones (such as testosterone) organize or alter the development of the mammalian brain to bring about its sexual and neuroanatomical differentiation. Therefore, the studies of this proposal's first section will attempt to elucidate the mechanism(s) of steroid action on the brain by investigating the effects of testosterone on the development of immature, male brain tissue after its transplantation into the brain of recipient female neonates. Particular emphasis will be given to possible gonadal steroid effects on the survival of transplanted neurons, their neuroanatomical structure, and their potential for migration. As a challenge for brain tissue transplants to alter the neural functions of recipient animals, the studies of this proposal's second section will test the ability of transplanted male brain tissue to establish functional connectivity with the host female brains, as evidenced by transplant-induced alterations in the recipient animals' sexual behavior and Luteinizing Hormone secretory patterns. If such modifications in brain functions are observed, strong evidence will have been provided not only for the functional integration of transplanted tissue, but for the feasibility of using brain tissue transplants clinically to restore normal function to damaged or diseased brain areas. The studies of this proposal involve a new intraparenchymal transplantation technique designed to discretely place transplanted brain tissue intothe most natural environment for its normal development and establishment of functional connectivity. As such, this proposal represents the first full-scale attempt to investigate the development and function of brain reproductive mechanisms through the use of brain tissue transplantation.

虽然脑组织移植在大鼠最近已被用于

项目成果

Labeling of neural cells by gold-filled Sendai virus envelopes before intracerebral transplantation.

脑内移植前用装金仙台病毒包膜标记神经细胞。

• DOI: 10.1126/science.2829355
• 发表时间: 1988
• 期刊: Science (New York, N.Y.)
• 作者: Ardizzoni,SC;Michaels,A;Arendash,GW
• 通讯作者: Arendash,GW

Transplantation of nucleus basalis magnocellularis cholinergic neurons into the cholinergic-depleted cerebral cortex. Morphological and behavioral effects.

将大细胞基底核胆碱能神经元移植到胆碱能耗尽的大脑皮层中。

• DOI: 10.1111/j.1749-6632.1987.tb23692.x
• 发表时间: 1987
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Arendash,GW;Mouton,PR
• 通讯作者: Mouton,PR

Induction of cortical cholinergic hypofunction and memory retention deficits through intracortical AF64A infusions.

通过皮质内 AF64A 输注诱导皮质胆碱能功能减退和记忆保留缺陷。

• DOI: 10.1016/0006-8993(88)90918-3
• 发表时间: 1988
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Mouton,PR;Meyer,EM;Dunn,AJ;Millard,W;Arendash,GW
• 通讯作者: Arendash,GW

Testosterone-induced enhancement of male medial preoptic tissue transplant volume in female recipients: a possible neuronotrophic action.

睾酮诱导的女性受者男性内侧视前组织移植体积的增强：可能的神经营养作用。

• DOI: 10.1016/0165-3806(87)90196-9
• 发表时间: 1987
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Arendash,GW;Gorski,RA
• 通讯作者: Gorski,RA

Alleviation of estrogen-induced hyperprolactinemia through intracerebral transplantation of hypothalamic tissue containing dopaminergic neurons.

通过脑内移植含有多巴胺能神经元的下丘脑组织来缓解雌激素引起的高催乳素血症。

• DOI: 10.1159/000124551
• 发表时间: 1986
• 期刊: Neuroendocrinology
• 影响因子: 4.1
• 作者: Arendash,GW;Leung,PC
• 通讯作者: Leung,PC