OPTIMIZING BREEDING EFFICIENCY IN THE DOMESTIC DOG

优化家犬的繁殖效率

• 批准号: 3450558
• 负责人: JOHN A PHILLIPS
• 金额: $ 14.43万
• 依托单位: ZOOLOGICAL SOCIETY OF SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3450558
• 关键词: bromocriptine; drug delivery systems; early diagnosis; estrogens; follicle stimulating hormone; gonadotropin releasing factor; high performance liquid chromatography; hormone regulation /control mechanism; luteinizing hormone; ovulation time; pregnancy; pregnancy tests; prenatal diagnosis; progesterone; prostaglandin F; radiotracer; reproduction; sex cycle

The current social trend against the use of random source dogs in biomedical research indicates that efficient methods of reproducing domestic dogs for laboratory purposes are urgently needed. New methods should be investigated which would augment controlled breeding since spontaneous natural mating occurs only one or two times a year in the domestic bitch, and previous attempts to induce this event have produced variable results. The present proposal is directed toward four objectives: 1) the induction of ovulation by utilizing a regimen of repetitive pulses of gonadotropin releasing hormone; 2) characterization of endogenous gonadotropin pulse patterns throughout the reproductive cycle; 3) an abbreviation of the normal metestrous period and attenuating the post-lactational effects of prolactin with treatments of prostaglandin F2Alpha and bromergocryptine, respectively; and 4) the early detection of pregnancy by quantifying plasma estrogen levels using an in vitro, cytosol receptor-binding assay for total estrogens. These studies will provide critical baseline information relating to spontaneous ovulation in the bitch while establishing the utility of gonadotropin releasing hormone as a treatment for advancing ovulation and optimizing conception. Additional information will be obtained which pertains to the mechanisms associated with luteal function and lactation by two separate strategies which are designed to further shorten the interbirth interval. These studies will be documented by hormonal data. Finally, a retrospective analysis of pregnant and non-pregnant, post-estrus bitches will investigate the potential of a chemical test for detecting pregnancy. Preliminary studies by the applicants demonstrate that a novel strategy to induce ovulation can be successfully employed in the domestic dog. Although not yet well-developed, this approach provides the colony manager with the ability to successfully breed anestrous bitches at will.

当前反对使用随机来源的狗的社会趋势 生物医学研究表明，高效的繁殖方法 迫切需要实验室用的家犬。新方法 应该进行调查，这将加强受控繁殖，因为 自然交配一年只发生一到两次 国内婊子，以及之前诱导这一事件的尝试 结果是可变的。 目前的建议是针对四个目标：1)归纳 通过重复使用促性腺激素脉冲治疗排卵 释放激素；2)内源性促性腺激素脉冲的特性 整个生殖周期的模式；3)的缩写 正常的发情期和减轻哺乳后的影响 催乳素联合前列腺素F2pha和溴隐亭治疗， 和4)通过定量血浆进行妊娠的早期检测 用体外胞浆受体结合法测定总雌激素水平 雌激素。 这些研究将提供与以下方面有关的关键基线信息 在母犬自发排卵的同时建立了 促性腺激素释放激素治疗促排卵和 优化观念。将获得其他信息，这些信息 与黄体功能和哺乳有关的机制 两个独立的战略，旨在进一步缩短 分娩间隔。这些研究将通过荷尔蒙数据进行记录。 最后，对妊娠与非妊娠、动情后的情况进行了回顾分析 母狗将调查化学测试的潜力，以检测 怀孕了。 申请者的初步研究表明，一种新的战略 诱导排卵可成功应用于家犬。 虽然还没有很好地开发，但这种方法为殖民地管理者提供了 有能力随心所欲地成功繁殖无性欲的母犬。