NMR MICROSCOPY & LOCALIZED SPECTROSCOPY AT 360 & 200 MHZ

这项提议的目标是发展核磁共振 360 MHz(核磁共振)显微镜和空间分辨光谱 (8.5T/8.9 cm口径)和200 MHz(4.7T/33 cm口径)， 提取的组织，以及有或没有肿瘤和 从而评估其可行性和适用性。细胞质 大单胞菌的特性及细胞动力学和代谢 细胞(超过100微米)，特别是青蛙卵，将是 在体内受精前后进行1H研究。 在以下条件下对镜片进行成像和光谱研究 正常和高糖培养条件将有所改善 对糖尿病和白内障发病机制的理解，因为它的影响 糖尿病对晶状体的影响反映了它对其他 纸巾。小动物身上的肿瘤将用显微镜检查 和局域光谱及其在肿瘤诊断中的意义 因此解决了异质性问题。从这些研究中， 肿瘤的形态和光谱特征 将被植入动物体内，并将其影响与 在癌症诊断方面进行了调查。分辨率限制 在200 MHz仪器上定义的大鼠和兔(包括和 没有植入肿瘤)将决定 显微镜观察更大的系统，特别是人类。 之所以选择这些研究中的生物系统，是因为它们 都有明确的定义，目前正在接受这一调查 实验室，以及这项技术的应用领域 将在他们的理解和临床上有很大的潜力 申请。这项建议的具体目标是(1) 获得可能的最佳分辨率并定义空间范围 核磁共振显微镜在不同大小的两个视野下的分辨率 生物系统；(2)对单个细胞进行核磁共振显微镜检查 并研究细胞新陈代谢、动力学(分裂)和 划分；(3)确定解决问题的能力 完整的活组织(晶状体)中细胞团的结构和 小动物(有肿瘤)；(4)实施31P和1H 小分子激光成像区的空间分辨光谱 动物、晶状体和细胞(仅限1H)，并定义 分辨率极限；以及(5)获得空间分辨弛豫 所选的时间(T1和T2)和自扩散系数 学习。

The goal of this proposal is to develop nuclear magnetic resonance (NMR) microscopy and spatially resolved spectroscopy at 360 MHz (8.5 T/8.9cm bore) and 200MHz (4.7T/33cm bore) on single cells, extracted tissues, and small animals with and without tumors and thus assess their feasibility and applicability. The cytoplasmic characteristics and cell dynamics and metabolism of large single cells (more than 100 microns), specifically frogs eggs, will be examined by 1H studies before and after fertilization in vivo. Imaging and spectrosocopic studies conducted on lenses under normal and high glucose media conditions will improve understanding of diabetes and cataractogenesis, since the effects of diabetes on the lens are reflective of its effect on other tissues. Tumors on small animals will be examined by microscopy and localized spectroscopy and the significance of tumor heterogeneity thus addressed. From these studies the morphologic and spectroscopic characteristics of tumors implanted in animals will be obtained and their implications with respect to cancer diagnosis investigated. The resolution limits defined on the 200MHz instrument on rats and rabbits (with and without implanted tumors) will determine the applicability of microscopy to larger systems, specifically humans. The biological systems in these studies are selected because they are well defined, are currently being investigated in this laboratory, and are areas in which application of this technology will have great potential in their understanding and clinical application. The specific objectives of this proposal are (1) to obtain the best possible resolution and define the limits of spatial resolution with NMR microscopy at both fields on different sized biological systems; (2) to perform NMR microscopy on single cells and study cell metabolism, dynamics (division) and compartmentalization; (3) to determine the capability of resolving the structure of cell clusters in intact living tissues (lenses) and small animals (with tumors); (4) to implement 31P and 1H spatially resolved spectroscopy on imaged regions of small animals, lenses and cells (1H only) at both fields and define the resolution limits; and (5) to obtain spatially resolved relaxation times (T1 and T2) and self-diffusion coefficients in selected studies.