STRUCTURE/FUNCTION OF TRANSCRIPTION COMPLEX RNA HAIRPINS
转录复合物 RNA 发夹的结构/功能
基本信息
- 批准号:3466376
- 负责人:
- 金额:$ 10.09万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1987
- 资助国家:美国
- 起止时间:1987-07-01 至 1992-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The goal of this project is to define the mechanisms by which
formation of secondary structures (RNA hairpins) in nascent
transcripts causes pausing and termination during transcription.
RNA secondary structures are important regulatory signals in
prokaryotes where transcription pausing and termination are
major components of genetic regulatory mechanisms; pausing and
premature termination also occur in eukaryotes where their
regulatory significance is only now emerging. The proposed
studies will provide a detailed understanding of how RNA hairpins
form and how they interact in the transcription complex.
Understanding this model systems will be an important
contribution to knowledge about transcription regulation, the
basic process that governs all gene expression. These studies also
will refine techniques and concepts that will be employed in
future work on eukaryotic transcription complexes.
A combination of biochemical, genetic, and recombinant DNA
techniques will be used to study RNA hairpin-transcription
complex interactions. Systematic variation of model RNA
hairpins will define the sequence and structural requirements for
induction of transcription pausing and termination by RNA hairpin
formation. A major goal will be to determine if the formation of
RNA hairpins causes pausing and termination only by altering
nucleic acid conformations in the transcription complex or if
specific interactions between proteins in the transcription
complex and portions of the RNA hairpin are involved. RNA
hairpin-specifying regions will be constructed from synthetic
oligonucleotides and inserted into special plasmid vectors in front
of the T7 A1 promoter to facilitate study of their pausing and
termination phenotypes. Transcription complexes containing
defined nascent transcript RNA hairpins will be isolated from in
vitro transcription reactions and subjected to extensive structural
analyses using enzymatic and chemical probes of RNA and DNA
structure. The beta subunit of RNA polymerase will be analyzed
by site-directed mutagenesis of its gene to determine which
amino acid residues may participate in RNA hairpin interactions.
这个项目的目标是定义这样的机制
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Robert Landick其他文献
Robert Landick的其他文献
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{{ truncateString('Robert Landick', 18)}}的其他基金
Diversity Supplement to Structure/Function of Transcription Complex Regulation to Support Predoctoral Student Christiana Binkley
转录复合体调节结构/功能的多样性补充以支持博士生克里斯蒂娜·宾克利
- 批准号:
10351034 - 财政年份:2020
- 资助金额:
$ 10.09万 - 项目类别:
Structure/Function of Transcription Complex Regulation
转录复合物调控的结构/功能
- 批准号:
7988507 - 财政年份:2009
- 资助金额:
$ 10.09万 - 项目类别:
Human RNAPII Structure/Function in Pausing & Elongation
暂停中的人类 RNAPII 结构/功能
- 批准号:
7391053 - 财政年份:2006
- 资助金额:
$ 10.09万 - 项目类别:
Human RNAPII Structure/Function in Pausing & Elongation
暂停中的人类 RNAPII 结构/功能
- 批准号:
7094722 - 财政年份:2006
- 资助金额:
$ 10.09万 - 项目类别:
Human RNAPII Structure/Function in Pausing & Elongation
暂停中的人类 RNAPII 结构/功能
- 批准号:
7211464 - 财政年份:2006
- 资助金额:
$ 10.09万 - 项目类别:
Human RNAPII Structure/Function in Pausing & Elongation
暂停中的人类 RNAPII 结构/功能
- 批准号:
7609099 - 财政年份:2006
- 资助金额:
$ 10.09万 - 项目类别:
High-Throughput Identification of RNA polymerase inhibitors in vivo
体内 RNA 聚合酶抑制剂的高通量鉴定
- 批准号:
7169444 - 财政年份:2006
- 资助金额:
$ 10.09万 - 项目类别:
Structure/Function of Transcription Complex RNA Hairpins
转录复合物RNA发夹的结构/功能
- 批准号:
6400682 - 财政年份:1987
- 资助金额:
$ 10.09万 - 项目类别:
STRUCTURE/FUNCTION OF TRANSCRIPTION COMPLEX RNA HAIRPINS
转录复合物 RNA 发夹的结构/功能
- 批准号:
3295256 - 财政年份:1987
- 资助金额:
$ 10.09万 - 项目类别:
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