MAMMALIAN DNA SEQUENCE MAPPING CENTER

哺乳动物 DNA 序列作图中心

• 批准号: 3487648
• 负责人: JOHN C HOZIER
• 金额: $ 5万
• 依托单位: GENETICS DATA SERVICES, INC.
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-30 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3487648
• 关键词: DNA; DVD /CD ROM; autoradiography; biomedical automation; chromosomes; computer graphics /printing; computers; genetic library; genetic mapping; genetic registry /resource /referral center; human tissue; image processing; information systems; molecular cloning; nucleic acid sequence; robotics; tissue /cell culture

Because of the rapidly increasing utilization of in situ DNA sequence mapping to mitotic chromosomes, it is proposed that a center be established to develop and employ the most efficient techniques and to automate the procedure. Such a center would make available to laboratories involved in the cloning of DNA sequences, the capability of efficiently mapping these sequences to mammalian chromosomes. DNA sequence by in situ hybridization would therefore be made available to laboratories that do not possess technical expertise in high resolution cytogenetics essential to this mapping technique. Conditions for in situ hybridization of molecular probes to mitotic chromosomes would be further developed, focusing on improvements in probe labelling protocols. Computer sutomated processing for in situ hybridization and automated data acquisition and analysis of autoradiographic grain distributions would be developed. It is proposed that Phase I activity be centered around the development of computer programs for analysis of grain distributions on chromosomes and studies of the availability of components for microcomputer-controlled automated hybridization procedures and autoradiographic procedures. Phase I activity would also include studies of the optimization of molecular probe labelling protocols focusing particularly on the use of 35S-RNA labelling. Phase II activity would include construction of an automated in situ hybridization system using microcomputer-controlled robotics technology as well as enhancement of the computer analysis programs to provide archiving of gene sequence mapping data.

由于原位DNA的利用率迅速增加 序列映射到有丝分裂染色体，提出了 建立中心以发展和采用最有效的效率 技术并自动化该过程。 这样的中心会 向参与DNA克隆的实验室提供 序列，有效绘制这些的能力 哺乳动物染色体的序列。 原位DNA序列 因此，杂交将提供给实验室 没有高分辨率的技术专业知识 细胞遗传学对这种映射技术必不可少。 状况 分子探针与有丝分裂的原位杂交 染色体将进一步开发，重点 探针标签协议的改进。 计算机缝合 处理原位杂交和自动数据 摄取和分析自显影谷物 将开发分布。 建议将I期活动集中在 开发用于分析谷物的计算机程序 关于染色体的分布和有关可用性的研究 微型计算机控制的自动杂交组件 程序和自显影程序。 第一阶段的活动 还包括对分子优化的研究 探测标签协议，特别是用于使用 35S-RNA标记。 第二阶段的活动将包括建造自动化的 使用微型计算机控制的原位杂交系统 机器人技术以及计算机的增强 分析程序提供基因序列映射的归档 数据。