QUANTITATION OF ESTROGEN RECEPTOR CONCENTRATION IN CELLS

该项目的目标是开发定量雌激素受体。 用高分辨率数字图像显微镜测量浓度 技巧。这些技术将使直接测量受体 在一个细胞的基础上的浓度。这比起之前的 目前的生化分析只给出受体的平均值 集中精神。建议开发的定量分析将1) 允许测量受体浓度的异质性 在细胞群体中，以及2)在肿瘤群体中细胞的比例 雌激素受体高于一定浓度水平的药物。这个 抗雌激素受体单抗的研究进展 雌激素免疫细胞化学定位方法的研究进展 接收器可行。定量成像的最新进展 细胞学可以与此相结合，发展一种快速、高效和更多的 准确的测定方法和消除视觉主观成分的方法 该方法。这些新技术的结合产生了一种新的 定量分析尤其重要，因为它已经被 研究表明，肿瘤中含有雌激素受体的患者将会有 对激素治疗有较高反应的可能性较高，似乎有 比那些肿瘤中不含这种受体的患者预后更好。

The objective of this project is to develop quantitative estrogen receptor concentration measurements using high resolution digital image microscopy techniques. These techniques will enable direct mesurements of receptor concentration on a cell by cell basis. This is an improvement over the current biochemical assays which only give an average value for receptor concentration. The quantitative assay proposed for development will 1) allow for a measurement of the heterogeneity of concentration of receptors in cell populations, and 2) the proportion of cells in a tumor population that have estrogen receptor above a certain concentration level. The recent development of monoclonal antibodies against estrogen receptor makes the development of methods for immunocytochemical localization of estrogen receptor feasible. Additional recent advances in quantitative imaging cytology can be combined wih this to develop a fast, efficient and more accurate assay method and to eliminate the visual subjective components of the method. This combination of these new techniques to produce a new quantitative assay is particularly important since it has already been shown that patients whose tumors contain estrogen receptors will have a higher probability of responding to hormonal therapy, and seem to have a better prognosis than patients whose tumors do not contain this receptor.