QUANTITATION OF ESTROGEN RECEPTOR CONCENTRATION IN CELLS

本项目的目的是建立定量的雌激素受体 使用高分辨率数字图像显微镜进行浓度测量 技术. 这些技术将能够直接介导受体 浓度在细胞的基础上。 这是一个改进， 目前的生物化学测定仅给出受体的平均值 浓度. 拟定用于开发的定量测定将1） 允许测量受体浓度的异质性 在细胞群体中，以及2）肿瘤群体中细胞的比例 雌激素受体的浓度高于一定水平。 的 雌激素受体单克隆抗体的发展， 雌激素免疫细胞化学定位方法的研究进展 受体可行。 定量成像的其他最新进展 细胞学可以与此相结合，以开发一种快速，高效， 准确的测定方法，并消除视觉主观成分 了该方法 这些新技术的结合， 定量分析是特别重要的，因为它已经被 显示肿瘤中含有雌激素受体的患者， 更高的概率响应激素治疗，似乎有一个 比肿瘤中不含这种受体的患者预后更好。

The objective of this project is to develop quantitative estrogen receptor concentration measurements using high resolution digital image microscopy techniques. These techniques will enable direct mesurements of receptor concentration on a cell by cell basis. This is an improvement over the current biochemical assays which only give an average value for receptor concentration. The quantitative assay proposed for development will 1) allow for a measurement of the heterogeneity of concentration of receptors in cell populations, and 2) the proportion of cells in a tumor population that have estrogen receptor above a certain concentration level. The recent development of monoclonal antibodies against estrogen receptor makes the development of methods for immunocytochemical localization of estrogen receptor feasible. Additional recent advances in quantitative imaging cytology can be combined wih this to develop a fast, efficient and more accurate assay method and to eliminate the visual subjective components of the method. This combination of these new techniques to produce a new quantitative assay is particularly important since it has already been shown that patients whose tumors contain estrogen receptors will have a higher probability of responding to hormonal therapy, and seem to have a better prognosis than patients whose tumors do not contain this receptor.