IMPROVED METHODS OF RED CELL MEASUREMENT PHASE II

红细胞测量第二阶段的改进方法

• 批准号: 3508342
• 负责人: JAMES W BACUS
• 金额: $ 15.42万
• 依托单位: CELL ANALYSIS SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1986-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508342
• 关键词: anemia; cell sorting; cell type; clinical biomedical equipment; densitometry; erythrocyte count; erythrocytes; hemoglobin; image processing; methemoglobin; oxyhemoglobin; spectrometry; visible light

The long term objective of this research is to apply new techniques of digital image processing to the analysis of red blood cells. It has been demonstrated, in our preliminary and related research, that these techniques have the potential of precise and rapid measurements of individual red cells with regard to their hemoglobin content, size, shape and central pallor. Further, subpopulations of cells can be rigorously defined according to characteristic variations of these measurements. These techniques could provide a new investigative tool for clinical medicine, resulting in enhanced, more efficient, anemia diagnosis. The primary objectives of these (Phase I) studies are to determine the optimum measurement wavelength, Lambda and associated extinction coefficient, Epsilon Lambda for the measurement of hemoglobin in dried red blood cells on a blood film. These are important constants, necessary as final design components for commercial application of the measurement techniques. These parameters will be selected with due consideration for the different types of hemoglobin present in the red cell and the correspondence between EpsilonLambda for solutions of hemoglobin. The potential commercial application of the proposed research is in the area of clinical laboratory blood analysis, instrumentation and testing.

这项研究的长期目标是应用新技术， 数字图像处理用于红细胞的分析。 已经 在我们的初步研究和相关研究中， 技术具有精确和快速测量的潜力， 单个红细胞的血红蛋白含量、大小、形状 和中心苍白 此外，细胞的亚群可以被严格地 根据这些测量的特征变化来定义。 这些技术可以为临床提供一种新的研究工具， 药物，从而增强，更有效，贫血诊断。 的 这些（I期）研究的主要目标是确定最佳 测量波长、λ和相关消光系数， 用于测量干红细胞中血红蛋白的Epa Lambda 在血膜上。 这些都是重要的常数，作为最终设计所必需的 用于测量技术的商业应用的组件。 这些 在选择参数时，将适当考虑不同类型 红细胞中血红蛋白的含量以及 血红蛋白溶液的ε λ。 潜在的商业 该研究的应用是在临床实验室领域 血液分析仪器和检测