QUANTITATION OF ESTROGEN RECEPTOR CONCENTRATION IN CELLS

本项目的目标是开发定量雌激素 使用高分辨率数字化的受体浓度测量 成像显微镜技术。 这些技术将使直接 组织和细胞中受体浓度的测量。 这 是对现有生化测定的改进， 给出了一个受体浓度的平均值， 均质化的样本样品可能含有不同的 恶性细胞的比例。 拟定试验将1） 测量细胞群中受体的浓度，2） 肿瘤细胞中含有雌激素的细胞比例 在一定浓度水平以上。 近期 雌激素受体单克隆抗体的研制 使得免疫细胞化学方法的发展 雌激素受体定位可行。 最近新增 定量成像细胞学的进展可以与 从而开发出一种快速、高效、准确的检测方法 并消除该方法的视觉主观成分。 这些新技术的结合， 定量分析是特别重要的，因为它已经 研究表明，肿瘤中含有雌激素受体的患者 对激素治疗有反应的可能性更高， 并且似乎比那些肿瘤 不含这种受体。

The objective of this project is to develop quantitative estrogen receptor concentration measurements using high resolution digital image microscopy techniques. These techniques will enable direct measurements of receptor concentration in tissue and cells. This is an improvement over the current biochemical assays which only give an average value for receptor concentration in a homogenized specimen sample which may contain varying proportions of malignant cells. The assay proposed will 1) measure the concentration of receptors in cell populations, 2) the proportion of cells in a tumor population that have estrogen receptor above a certain concentration level. The recent development of monoclonal antibodies against estrogen receptors makes the development of methods for immunocytochemical localization of estrogen receptors feasible. Additional recent advances in quantitative imaging cytology can be combined with this to develop a fast, efficient and more accurate assay method and to eliminate the visual subjective components of the method. This combination of these new techniques to produce a new quantitative assay is particularly important since it has already been shown that patients whose tumors contain estrogen receptors will have a higher probability of responding to hormonal therapy, and seem to have a better prognosis than patients whose tumors do not contain this receptor.