HORMONAL AND IONIC CONTROL OF METABOLISM

新陈代谢的激素和离子控制

• 批准号: 3483318
• 负责人: HOWARD RASMUSSEN
• 金额: $ 26.73万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1995-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3483318
• 关键词: 3T3 cells; Gastropoda; adrenal glands; adrenocorticotropic hormone; angiotensin II; biological information processing; brush border membrane; calcium channel; calcium metabolism; calmodulin; cholera toxin; cow; cyclic AMP; cytoplasm; drug receptors; electron microscopy; erythrocyte membrane; gastrointestinal absorption /transport; gel filtration chromatography; glucose transport; hormone regulation /control mechanism; human tissue; hydrogen; intestinal mucosa; intracellular transport; ion transport; ionophores; laboratory rabbit; laboratory rat; liver cells; membrane transport proteins; mitochondria; muscle contraction; myosins; peptide hormone; phenothiazines; radiotracer; sodium

The major aims of the proposed research is to more fully define the mechanisms by which CA2+ regulates cell function during such processes as the hormonal and ionic regulations of aldosterone secretion, from adrenal glomerulosa cells. Cell proliferation of cultured 3T3 cells, and leukocytes function. Specific aims include an elucidation of the temporal patterns of protein phosphorylation, inositol lipid metabolism, Ca2+ metabolism, and protein kinase C function during angiotensin II and bombesin-mediated response in, respectively, glomerulosa and 3T3 cells. The role of type II CaM-dependent protein kinase in the initial phase of cellular response; the immunocytochemical localization of the C-kinase and the possible role of M-kinase in sustained cellular response; the mechanism by which information is conveyed from cell surface to cell interior via the C-kinase branch of the Ca2+ messenger system during the sustained phase of cellular response focusing particularly on changes in Na+/H+ exchange and possible protein kinase cascades; the molecular basis of memory in the C-kinase-mediated phase of cellular response; and a reexamination of the temporal pattern of events in the cAMP messenger system during ACTH-mediate aldosterone secretion from adrenal glomerulosa cells as the system in which to explore these issues. Methods will include studies of Ca2+ fluxes and measurements of intracellular free Ca2+ using both fura 2 and aequorin as indicators; studies of the turnover of inositol lipids and inositols phosphates by both chemical and radio- isotope methods; 2-D gel electrophoresis of the phosphoproteins obtained from extracts of cells; and both light and EM immunocytochemical localization of C-kinase. The problem under investigation is of broad biological interest, and has implications for a wide range of biological phenomena. A better understanding of Ca2+ messenger system function is likely to alter our views of cell growth and proliferation, peptide and steroid hormone secretion, and smooth muscle contraction.

拟议研究的主要目的是更全面地界定 CA 2+调节细胞功能的机制 这些过程，如激素和离子调节， 肾上腺小球细胞分泌醛固酮。 细胞 培养的3 T3细胞增殖和白细胞功能。 具体目标包括阐明 蛋白磷酸化，肌醇脂代谢，钙离子 血管紧张素II代谢和蛋白激酶C功能 和铃蟾肽介导的反应，分别在肾小球和 3 T3细胞。 II型钙调素依赖性蛋白激酶在糖尿病中的作用 细胞反应的初始阶段;免疫细胞化学 C-激酶的定位和M-激酶在 持续的细胞反应;信息传递的机制 通过C-激酶从细胞表面传递到细胞内部 持续期钙信使系统的分支 细胞反应特别关注Na+/H+ 交换和可能的蛋白激酶级联;分子 C-激酶介导的细胞记忆阶段的基础 反应;以及重新审查事件的时间模式 在ACTH介导的醛固酮释放过程中cAMP信使系统 肾上腺肾小球细胞分泌的系统，其中 来探讨这些问题。 方法将包括钙离子的研究 通量和测量细胞内游离Ca 2+， fura 2和水母发光蛋白作为指标; 肌醇脂质和肌醇磷酸盐的化学和放射性- 同位素法;磷蛋白质二维凝胶电泳 从细胞提取物中获得;光和EM C-激酶免疫细胞化学定位。 下的问题 研究具有广泛的生物学意义， 一系列的生物现象。 更好地理解 Ca 2+信使系统的功能可能会改变我们对 细胞生长和增殖，肽和类固醇激素 分泌和平滑肌收缩。