VISAGE/EQ ELECTROPHORESIS ANALYSIS SYSTEM

VISAGE/EQ电泳分析系统

• 批准号: 3519810
• 负责人: HOWARD RASMUSSEN
• 金额: $ 13.1万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-02-20 至 1988-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519810
• 关键词: autoradiography; densitometry; gel electrophoresis; image processing; lipids; phosphopeptides; thin layer chromatography

This is a request for a shared instrument which will be employed largely as a means of carrying out quantitative analyzes of autoradiographs from 2-D gels phosphoprotein electrophoresis patterns. The request is being submitted by a group of 17 independent investigators on the faculty of Yale University School of Medicine in the departments of Cell Biology, Internal Medicine, Pharmacology, Pathology, Neurology and Surgery. The use of the instrument will be controlled by an interdepartmental advisory group, and will be housed in space provided by the Department of Cell Biology. Members of the user group will oversee its use of the instrument and maintain and house the instrument. the major two uses of the instrument will be the analysis of autoradiographs of phosphoproteins and phosphopeptides, and the analysis, by a reflectance method, of lipids on thin layer plates.

这是一个共同文书的请求， 主要是作为一种手段，进行定量分析， 二维凝胶磷蛋白电泳放射自显影 模式. 这项请求是由一个17人小组提出的， 耶鲁大学学院的独立调查员 在细胞生物学，内科， 药理学、病理学、神经病学和外科学。 的使用 仪器将由一个跨部门咨询机构控制。 小组，并将被安置在空间提供的部门， 细胞生物学 用户组的成员将监督其使用 仪器并维护和存放仪器。 主要 该仪器的两个用途是分析放射自显影 磷蛋白和磷肽的分析，以及通过 薄层板上脂质的反射法。