HORMONAL AND IONIC CONTROL OF METABOLISM

新陈代谢的激素和离子控制

• 批准号: 2137386
• 负责人: HOWARD RASMUSSEN
• 金额: $ 14.92万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2137386
• 关键词: 3T3 cells; Gastropoda; adrenal glands; adrenocorticotropic hormone; angiotensin II; biological information processing; brush border membrane; calcium channel; calcium metabolism; calmodulin; cholera toxin; cow; cyclic AMP; cytoplasm; drug receptors; electron microscopy; erythrocyte membrane; gastrointestinal absorption /transport; gel filtration chromatography; glucose transport; hormone regulation /control mechanism; human tissue; hydrogen; intestinal mucosa; intracellular transport; ion transport; ionophores; laboratory rabbit; laboratory rat; liver cells; membrane transport proteins; mitochondria; muscle contraction; myosins; peptide hormone; phenothiazines; radiotracer; sodium

The major aims of the proposed research is to more fully define the mechanisms by which CA2+ regulates cell function during such processes as the hormonal and ionic regulations of aldosterone secretion, from adrenal glomerulosa cells. Cell proliferation of cultured 3T3 cells, and leukocytes function. Specific aims include an elucidation of the temporal patterns of protein phosphorylation, inositol lipid metabolism, Ca2+ metabolism, and protein kinase C function during angiotensin II and bombesin-mediated response in, respectively, glomerulosa and 3T3 cells. The role of type II CaM-dependent protein kinase in the initial phase of cellular response; the immunocytochemical localization of the C-kinase and the possible role of M-kinase in sustained cellular response; the mechanism by which information is conveyed from cell surface to cell interior via the C-kinase branch of the Ca2+ messenger system during the sustained phase of cellular response focusing particularly on changes in Na+/H+ exchange and possible protein kinase cascades; the molecular basis of memory in the C-kinase-mediated phase of cellular response; and a reexamination of the temporal pattern of events in the cAMP messenger system during ACTH-mediate aldosterone secretion from adrenal glomerulosa cells as the system in which to explore these issues. Methods will include studies of Ca2+ fluxes and measurements of intracellular free Ca2+ using both fura 2 and aequorin as indicators; studies of the turnover of inositol lipids and inositols phosphates by both chemical and radio- isotope methods; 2-D gel electrophoresis of the phosphoproteins obtained from extracts of cells; and both light and EM immunocytochemical localization of C-kinase. The problem under investigation is of broad biological interest, and has implications for a wide range of biological phenomena. A better understanding of Ca2+ messenger system function is likely to alter our views of cell growth and proliferation, peptide and steroid hormone secretion, and smooth muscle contraction.

拟议研究的主要目的是更全面地定义

项目成果

Calcium as intracellular messenger: from simplicity to complexity.

• DOI: 10.1016/b978-0-12-152831-7.50003-2
• 发表时间: 1990
• 期刊: Current topics in cellular regulation
• 作者: H. Rasmussen;J. Rasmussen

Ca(2+)-cyclic AMP interactions in sustained cellular responses.

• DOI: 10.1002/9780470514207.ch7
• 发表时间: 1992
• 期刊: Ciba Foundation symposium
• 作者: H. Rasmussen;C. Isales;S. Ganesan;R. Calle;W. Zawalich

Influence of staurosporine on glucose-mediated and glucose-conditioned insulin secretion.

星形孢菌素对葡萄糖介导和葡萄糖调节的胰岛素分泌的影响。

• DOI: 10.1042/bj2790807
• 发表时间: 1991
• 期刊: The Biochemical journal
• 作者: Zawalich,WS;Zawalich,KC;Ganesan,S;Calle,R;Rasmussen,H
• 通讯作者: Rasmussen,H

Expression of inducible nitric oxide synthase in breast cancer correlates with metastatic disease.

• 发表时间: 1997-07
• 期刊: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
• 作者: Alfonso Dueñas-González;Carlos M. Isales;M. M. Abad-Hernández-M.;Rogelio González-Sarmiento;O. Sangueza;J. Rodriguez-Commes

ANPs effect on MARCKS and StAR phosphorylation in agonist-stimulated glomerulosa cells.

ANPs 对激动剂刺激的肾小球细胞中 MARCKS 和 StAR 磷酸化的影响。

• DOI: 10.1016/s0303-7207(01)00454-3
• 发表时间: 2001
• 期刊: Molecular and cellular endocrinology
• 影响因子: 4.1
• 作者: Calle,RA;Bollag,WB;White,S;Betancourt-Calle,S;Kent,P
• 通讯作者: Kent,P